Thermo Scientific™

CaptureSelect™ FcXL Pre-packed Column

Catalog number:  494328001

 Related applications:


Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}} also known as {{product.formattedSku}} 
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top


CaptureSelect™ FcXL Affinity Matrix, available here in a pre-packed column format, has been specifically designed for the purification of recombinant human IgG, Fc fusion proteins, and plasma derived IgG. It recognizes the CH3 domain of all human IgG subclasses (IgG1, 2, 3, and 4).

Features of this affinity matrix include:
• Mild elution, making it suitable for Fc fusion proteins
• Human specific (e.g., no binding to bovine antibodies)
• Excellent scalability
• Non-animal-derived

CaptureSelect™ FcXL Affinity Matrix purifies IgGs and Fc fusion proteins directly from complex source materials in a single step with high purity and yield.

Free of animal components
CaptureSelect™ products are affinity ligands created by a proprietary technology based on Camelid-derived single-domain antibody fragments. The ligand is a 13 kDa single-domain fragment comprising the 3 CDRs that form the antigen binding domain, efficiently produced by the yeast Saccharomyces cerevisiae in a production process free of animal components.


Matrix: agarose-based, aldehyde-activated
Average particle size: 65 ± 10 µm
Ligand: CaptureSelect™ FcXL affinity ligand
Ligand coupling method: aldehyde coupling via NH2 residues of the ligand
Binding capacity: ~25 g IgG/L resin at 5-10 minutes contact time
Elution conditions: 20 mM citric acid or acetic acid, pH 3–4, or 20 mM sodium acetate, 1.0 M MgCl2, 40% (v/v) propylene glycol, pH 5–6
Flow characteristics: 150–300 cm/h
Formulation buffer: 20%(v/v) ethanol
For Research Use Only. Not for use in diagnostic procedures.


Column Type: Affinity Column
Format: Chromatography Cartridge
Immobilized Phase: Anti-IgG-Fc
Product Line: CaptureSelect™
Product Size: 5 x 1 mL columns
Shipping Condition: Approved for shipment at Room Temperature or on Wet Ice

Contents & storage

Store in refrigerator (2–8°C).


Manuals & protocols