CaptureSelect™ Pre-packed Column FcXL

Catalog number: 494328001

 Related applications: Chromatography

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Description

CaptureSelect™ FcXL Affinity Matrix, available here in a pre-packed column format, has been specifically designed for the purification of recombinant human IgG, Fc fusion proteins, and plasma derived IgG. It recognizes the CH3 domain of all human IgG subclasses (IgG1, 2, 3, and 4).

Features of this affinity matrix include:
• Mild elution, making it suitable for Fc fusion proteins
• Human specific (e.g., no binding to bovine antibodies)
• Excellent scalability
• Non-animal-derived

CaptureSelect™ FcXL Affinity Matrix purifies IgGs and Fc fusion proteins directly from complex source materials in a single step with high purity and yield.

Free of animal components
CaptureSelect™ products are affinity ligands created by a proprietary technology based on Camelid-derived single-domain antibody fragments. The ligand is a 13 kDa single-domain fragment comprising the 3 CDRs that form the antigen binding domain, efficiently produced by the yeast Saccharomyces cerevisiae in a production process free of animal components.

Characteristics:

Matrix: agarose-based, aldehyde-activated
Average particle size: 65 ± 10 µm
Ligand: CaptureSelect™ FcXL affinity ligand
Ligand coupling method: aldehyde coupling via NH2 residues of the ligand
Binding capacity: ~25 g IgG/L resin at 5-10 minutes contact time
Elution conditions: 20 mM citric acid or acetic acid, pH 3–4, or 20 mM sodium acetate, 1.0 M MgCl2, 40% (v/v) propylene glycol, pH 5–6
Flow characteristics: 150–300 cm/h
Formulation buffer: 20%(v/v) ethanol
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format: Chromatography Cartridge
Column Type: Affinity Column
Product Size: 5 x 1 mL columns
Immobilized Phase: Anti-IgG-Fc
Shipping Condition: Approved for shipment at Room Temperature or on Wet Ice

Contents & storage

Store in refrigerator (2–8°C).

Documents

Manuals & protocols