This product will be discontinued in December 2020. For more information and replacement products, please view the discontinuation letter.
Thermo Scientific™

CaptureSelect™ FcXL Pre-packed Column

Catalog number: 494328001
Thermo Scientific™

CaptureSelect™ FcXL Pre-packed Column

Catalog number: 494328001
Catalog Number
494328001
Unit Size
5 x 1 mL columns
Price (USD)
1,338.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
1 x 5 mL column
Price (USD)
1,338.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
4943280015 x 1 mL columns
1,338.00
Your Price:
***
4943280051 x 5 mL column
1,338.00
Your Price:
***
Product Overview
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CaptureSelect™ FcXL Affinity Matrix, available here in a pre-packed column format, has been specifically designed for the purification of recombinant human IgG, Fc fusion proteins, and plasma derived IgG. It recognizes the CH3 domain of all human IgG subclasses (IgG1, 2, 3, and 4).

Features of this affinity matrix include:
• Mild elution, making it suitable for Fc fusion proteins
• Human specific (e.g., no binding to bovine antibodies)
• Excellent scalability
• Non-animal-derived

CaptureSelect™ FcXL Affinity Matrix purifies IgGs and Fc fusion proteins directly from complex source materials in a single step with high purity and yield.

Free of animal components
CaptureSelect™ products are affinity ligands created by a proprietary technology based on Camelid-derived single-domain antibody fragments. The ligand is a 13 kDa single-domain fragment comprising the 3 CDRs that form the antigen binding domain, efficiently produced by the yeast Saccharomyces cerevisiae in a production process free of animal components.

Characteristics:

Matrix: agarose-based, aldehyde-activated
Average particle size: 65 ± 10 µm
Ligand: CaptureSelect™ FcXL affinity ligand
Ligand coupling method: aldehyde coupling via NH2 residues of the ligand
Binding capacity: ∼25 g IgG/L resin at 5-10 minutes contact time
Elution conditions: 20 mM citric acid or acetic acid, pH 3–4, or 20 mM sodium acetate, 1.0 M MgCl2, 40% (v/v) propylene glycol, pH 5–6
Flow characteristics: 150–300 cm/h
Formulation buffer: 20%(v/v) ethanol
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Quantity
5 x 1 mL
Shipping Condition
Approved for shipment at Room Temperature or on Wet Ice
Stationary Phase
Anti-IgG-Fc
Column Type
Affinity
Form
Chromatography Cartridge
Product Line
CaptureSelect™
Type
Column

Contents & Storage

Store in refrigerator (2–8°C).

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Frequently asked questions (FAQs)

Citations & References