BACE1 Recombinant Mouse Protein, His tag

Description

Beta-site APP-cleaving enzyme 1 (BACE1), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Thr 457) of the mouse BACE1 precursor (NM_011792.5) fused to thel polyhistidine tag at the C-terminus.

Activity: Measured by its ability to cleave a fluorescent peptide substrate Mca-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Arg-Lys(Dpn)-Arg-Arg-NH₂. Cleavage of this substrate can be measured using excitation and emission wavelengths of 320 and 405 nm, respectively.
The specific activity is >2 pmoles/min/µg.

Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na₂HPO₄, 1.8 mM KH₂PO₄, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by Sino Biological Inc.

Specifications

Protein Family:	Other Proteins
Research Category:	Neurobiology
Endotoxin Level:	< 1 EU/µg
Expression:	Mammalian
Form:	Lyophilized
Gene Aliases:	Bace1, C76936
Gene Symbol:	BACE1
Molecular Weight:	49.8 kDa
Product Line:	Sino Biological®
Product Size:	5 x 50 µg
Protein Name:	beta-site APP cleaving enzyme 1
Protein Subtype:	Proteases
Protein Tag or Fusion:	His Tag
Purity:	≥ 90% by SDS-PAGE
Species:	Mouse
Validated Application:	Assay Standard

Contents & storage