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BACE1 Recombinant Mouse Protein, His tag

Catalog number: 50002M08H5

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Description

Beta-site APP-cleaving enzyme 1 (BACE1), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Thr 457) of the mouse BACE1 precursor (NM_011792.5) fused to thel polyhistidine tag at the C-terminus.

Activity: Measured by its ability to cleave a fluorescent peptide substrate Mca-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Arg-Lys(Dpn)-Arg-Arg-NH2. Cleavage of this substrate can be measured using excitation and emission wavelengths of 320 and 405 nm, respectively.
The specific activity is >2 pmoles/min/µg.

Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by Sino Biological Inc.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Protein Family: Other Proteins
Research Category: Neurobiology
Endotoxin Level: < 1 EU/µg
Expression: Mammalian
Form: Lyophilized
Gene Aliases: Bace1, C76936
Gene Symbol: BACE1
Molecular Weight: 49.8 kDa
Product Line: Sino Biological®
Product Size: 5 µg
Protein Name: beta-site APP cleaving enzyme 1
Protein Subtype: Proteases
Protein Tag or Fusion: His Tag
Purity: ≥ 90% by SDS-PAGE
Species: Mouse
Validated Application: Assay Standard

Contents & storage

Includes 1 vial of lyophilized protein. Store at -20 °C.

Documents

Manuals & protocols