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IL1B Recombinant Mouse Protein

Catalog number: 50101MNAE5

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Description

Interleukin 1 Beta (IL1B), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications

This recombinant protein was expressed from a DNA sequence encoding the mature form of mouse IL1B (P10749) (Val 118-Ser 269) fused to an initial Met at the C-terminus.

Activity:
1. Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is typically 2-10 pg/ml.

2. Measured by its ability to bind human IL1R1 in a functional ELISA.

3. Measured by its ability to bind mouse IL1R1 in a functional ELISA.

Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by Sino Biological Inc.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Research Category: Immunology, Inflammation, Neurobiology, Signal Transduction
Expression: E. coli
Form: Lyophilized
Gene Aliases: RP23-384K11.2, IL-1beta, Il-1b
Gene Symbol: IL1B
Molecular Weight: 17.5 kDa
Product Line: Sino Biological®
Product Size: 5 µg
Protein Family: Cytokines & Receptors
Protein Name: Interleukin 1 beta
Protein Subtype: Interleukins
Protein Tag or Fusion: Untagged
Purity: ≥ 95% by SDS-PAGE
Species: Mouse
Validated Application: Assay Standard

Contents & storage

Includes 1 vial of lyophilized protein. Store at -20 °C.

Documents

Manuals & protocols

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