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CD28 Recombinant Mouse Protein, hIgG1-Fc.His tag

Catalog number: 50103M03H25

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Cluster of Differentiation 28 (CD28), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in studying protein-protein interactions, and cell biology research applications. It can also be used as a protein standard.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Lys 149) of mouse CD28 precursor (NP_031668.3) fused to the polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Activity: Measured by its binding ability in a functional ELISA .
1. Immobilized mouse CD86 at 20 µg/ml(100 µl/well) can bind mouse CD28 -Fc with a linear range of 6.4-800 ng/ml.
2. Immobilized rat CD86 at 2 µg/ml(100 µl/well) can bind mouse CD28-Fc with a linear range of 6.4-160 ng/ml.

Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by Sino Biological Inc.

For Research Use Only. Not for use in diagnostic procedures.


Form: Lyophilized
Purity: ≥ 90% by SDS-PAGE
Gene ID: 12487
Species: Mouse
Expression: Mammalian
Gene Symbol: CD28
Gene Aliases: Cd28, CH29-189K17.3
Product Size: 5 x 5 µg
Protein Form: Truncated, Recombinant, Cell Surface Protein
Protein Family: CD Proteins
Endotoxin Level: < 1 EU/µg
Protein Subtype: Glycoproteins
Molecular Weight: 43 kDa
Research Category: Immunology
Protein Tag or Fusion: His Tag, IgG1 Fc Tag
Validated Application: Assay Standard
Shipping Condition: Room Temperature

Contents & storage

Store at -20 to -70 °C


Manuals & protocols