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CD28 Recombinant Mouse Protein, hIgG1-Fc.His tag

Catalog number: 50103M03H250

 Related applications: Protein Assays and Analysis

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Description

Cluster of Differentiation 28 (CD28), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in studying protein-protein interactions, and cell biology research applications. It can also be used as a protein standard.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Lys 149) of mouse CD28 precursor (NP_031668.3) fused to the polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Activity: Measured by its binding ability in a functional ELISA .
1. Immobilized mouse CD86 at 20 µg/ml(100 µl/well) can bind mouse CD28 -Fc with a linear range of 6.4-800 ng/ml.
2. Immobilized rat CD86 at 2 µg/ml(100 µl/well) can bind mouse CD28-Fc with a linear range of 6.4-160 ng/ml.

Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.



This protein is manufactured by Sino Biological Inc.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Protein Family: CD Proteins
Research Category: Immunology
Endotoxin Level: < 1 EU/µg
Expression: Mammalian
Form: Lyophilized
Gene Aliases: Cd28, CH29-189K17.3
Gene ID: 12487
Gene Symbol: CD28
Molecular Weight: 43 kDa
Product Line: Sino Biological®
Product Size: 5 x 50 µg
Protein Form: Cell Surface Protein, Recombinant, Truncated
Protein Name: CD28 antigen
Protein Subtype: Glycoproteins
Protein Tag or Fusion: His Tag, IgG1 Fc Tag
Purity: ≥ 90% by SDS-PAGE
Species: Mouse
Validated Application: Assay Standard
Shipping Condition: Room Temperature

Contents & storage

Store at -20 to -70 °C

Documents

Manuals & protocols