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CD27 Recombinant Mouse Protein, His tag

Catalog number: 50110M08H25

 Related applications: Protein Assays and Analysis

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Description

Cluster of Differentiation 27 (CD27), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, studying protein-protein interactions, and cell biology research applications. It can also be used as an immunogen, or as a protein standard.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Arg 182) of mouse CD27 (NP_001028298.1) fused to a polyhistidine tag at the C-terminus.

Activity: Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse CD27 at 2 µg/ml(100 µl/well) can bind human CD70 with a linear range of 78-1250 pg/ml.

Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.



This protein is manufactured by Sino Biological Inc.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Endotoxin Level: < 1 EU/µg
Expression: Mammalian
Form: Lyophilized
Gene Aliases: S152, Tnfrsf7, Tp55
Gene ID: 21940
Gene Symbol: CD27
Molecular Weight: 19.6 kDa
Product Size: 5 x 5 µg
Protein Family: CD Proteins
Protein Form: Cell Surface Protein, Recombinant, Truncated
Protein Subtype: TNF Receptors
Protein Tag or Fusion: His Tag
Purity: ≥ 90% by SDS-PAGE
Research Category: Immunology, Signal Transduction
Species: Mouse
Validated Application: Assay Standard
Shipping Condition: Room Temperature

Contents & storage

Store at -20 to -70 °C

Documents

Manuals & protocols