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IL6ST Recombinant Mouse Protein, His Tag

Catalog number: 50135M08H250

 Related applications: Protein Assays and Analysis

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Interleukin 6 signal transducer (IL6ST), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and cell biology research applications. It can also be used as an immunogen, or as a protein standard.

This recombinant protein was expressed from a DNA sequence encoding the mouse extracellular domain (Met 1-Glu 617) of IL6ST (NP_034690.3) fused to a polyhistidine tag at the C-terminus.

1. Measured by its ability to bind mouse IL11RA in a functional ELISA.
2. Measured by its ability to inhibit the IL6R enhancement of IL6 activity on M1 mouse myeloid leukemia cells (Saito, T. et al. 1991, J. Immunol. 147:168). The ED50 for this effect is typically 0.2-0.8 µg/mlin the presence of 50 ng/mlrecombinant human IL6sR and 100 ng/mlrecombinant human IL6.

Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by Sino Biological Inc.

For Research Use Only. Not for use in diagnostic procedures.


Form: Lyophilized
Purity: ≥ 90% by SDS-PAGE
Gene ID: 16195
Species: Mouse
Expression: Mammalian
Gene Symbol: IL6ST
Gene Aliases: Il6st, 5133400A03Rik, AA389424, BB405851, CD130, D13Ertd699e, gp130
Product Size: 5 x 50 µg
Protein Form: Truncated, Recombinant
Protein Family: Cytokines & Receptors, CD Proteins
Endotoxin Level: < 1 EU/µg
Protein Subtype: Interleukins, Cytokine Receptors
Molecular Weight: 68 kDa
Research Category: Immunology, Oncology, Signal Transduction
Protein Tag or Fusion: His Tag
Validated Application: Assay Standard
Shipping Condition: Room Temperature

Contents & storage

Store at -20 to -70 °C


Manuals & protocols

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