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IGFBP4 Recombinant Mouse Protein, His Tag

Catalog number: 50250M08H250

 Related applications: Protein Assays and Analysis

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Description

Insulin-Like Growth Factor Binding Protein 4 (IGFBP4), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications

This recombinant protein was expressed from a DNA sequence encoding the human IGFBP4 (NP_001543.2) precursor (Met 1-Glu 258) fused to a C-terminal polyhistidine tag.

Activity: Measured in a serumfree cell proliferation assay using MCF7 human breast adenocarcinoma cells (Karey, K.P. et al. 1988, Cancer Research 48: 4083). The ED50 is typically 5-10 µg/ml.

Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by Sino Biological Inc.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Research Category: Bone Research, Oncology, Reproduction
Endotoxin Level: < 1 EU/µg
Expression: Mammalian
Form: Lyophilized
Gene Aliases: Igfbp4, RP23-333D2.6, AI875747, Deb2, IGFBP-4
Gene Symbol: IGFBP4
Molecular Weight: 27.2 kDa
Product Line: Sino Biological®
Product Size: 5 x 50 µg
Protein Family: Growth Factors & Receptors
Protein Name: insulin-like growth factor binding protein 4
Protein Subtype: Growth Factor Receptors, IGFs (Insulin-Like Growth Factors)
Protein Tag or Fusion: His Tag
Purity: ≥ 90% by SDS-PAGE
Species: Mouse
Validated Application: Assay Standard

Contents & storage

Includes 5 vial of lyophilized protein. Store at -20 °C.

Documents

Manuals & protocols

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