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NGF Recombinant Mouse Protein

Catalog number: 50385MNAC250

 Related applications: Protein Assays and Analysis

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Description

Nerve growth factor (NGF), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in performing cell based assays. It can also be used as a protein standard, or in other research applications.

This recombinant protein was expressed from a DNA sequence encoding the mature form of mouse NGF (NP_001106168.1), residues 122 to 241, with an initial Met at the N-terminus.

Activity: Measured in a cell proliferation assay using TF1 human erythroleukemic cells. The ED50 for this effec is 0.2-2.0 ng/ml.

Formulation: Lyophilized in 50 mM Na acetate buffer, pH 5.5, 150 mM NaCl, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.



This protein is manufactured by Sino Biological Inc.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Research Category: Development, Immunology, Inflammation, Neurobiology
Endotoxin Level: < 1 EU/µg
Expression: Mammalian
Form: Lyophilized
Gene Aliases: Ngf, Ngfb
Gene ID: 18049
Gene Symbol: NGF
Molecular Weight: 13.5 kDa
Product Line: Sino Biological®
Product Size: 5 x 50 µg
Protein Family: Growth Factors & Receptors
Protein Form: Native
Protein Name: nerve growth factor
Protein Subtype: Neurotrophic Factors
Protein Tag or Fusion: Untagged
Purity: ≥ 95% by SDS-PAGE
Species: Mouse
Validated Application: Assay Standard
Shipping Condition: Room Temperature

Contents & storage

Store at -20 to -70 °C

Documents

Manuals & protocols

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