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NGF Recombinant Mouse Protein

Catalog number: 50385MNAC5

 Related applications: Protein Assays and Analysis

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Nerve growth factor (NGF), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in performing cell based assays. It can also be used as a protein standard, or in other research applications.

This recombinant protein was expressed from a DNA sequence encoding the mature form of mouse NGF (NP_001106168.1), residues 122 to 241, with an initial Met at the N-terminus.

Activity: Measured in a cell proliferation assay using TF1 human erythroleukemic cells. The ED50 for this effec is 0.2-2.0 ng/ml.

Formulation: Lyophilized in 50 mM Na acetate buffer, pH 5.5, 150 mM NaCl, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by Sino Biological Inc.

For Research Use Only. Not for use in diagnostic procedures.


Form: Lyophilized
Purity: ≥ 95% by SDS-PAGE
Gene ID: 18049
Species: Mouse
Expression: Mammalian
Gene Symbol: NGF
Gene Aliases: Ngf, Ngfb
Product Size: 5 µg
Protein Form: Native
Protein Family: Growth Factors & Receptors
Endotoxin Level: < 1 EU/µg
Protein Subtype: Neurotrophic Factors
Molecular Weight: 13.5 kDa
Research Category: Immunology, Development, Neurobiology, Inflammation
Protein Tag or Fusion: Untagged
Validated Application: Assay Standard
Shipping Condition: Room Temperature

Contents & storage

Store at -20 to -70 °C


Manuals & protocols

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