Thermo Scientific™

UltiMate™ NCS-3500RS Binary Rapid Separation Nano/Capillary Pumps

Catalog number: 5041.0010A
Thermo Scientific™

UltiMate™ NCS-3500RS Binary Rapid Separation Nano/Capillary Pumps

Catalog number: 5041.0010A

Access the highest resolution, the fastest analyses and exceptional column identification flexibility in one module. The Thermo Scientific™ UltiMate™ NCS-3500RS Binary Rapid Separation Nano/Capillary Pumps combine a ternary gradient microloading pump, a binary nano/capillary low-flow pump and a heated column compartment in one housing.The integrated micro-flow loading pump accommodates pre-concentration workflows, advanced two-dimensional chromatography or even 2 to 4.6mm column inner diameter flows up to 62MPa (9,000 psi/ 620 bar). Our Thermo Scientific™ ProFlow™ technology improves nano flowrate control and results in high retention time precision and higher quality data. Achieve high-sensitivity with our nano-flow solution, or achieve high-sensitivity and high-throughput with robust capillary solutions.

 
Catalog Number
5041.0010A
Unit Size
Each
Description
NCS-3500RS Nano LC Pump with ProFlow Flow Meter and Column Compartment
Price (USD)
Full specifications
Min. Flow RateBinary High Pressure Gradient Pump: Nano ProFlow Flow Meter: 0 nL/min; Capillary Flow Meter: 0 μL/min; Micro Flow Meter: 0 μL/min;; Ternary Micro Pump: 0 μL/min
Operating Principlebinary high pressure gradient mixing pump (column) and ternary low pressure gradient mixing (trap-column loading)
Solvent DegassingExternal/Optional
Biocompatible VersionYes
Gradient FormationHigh-Pressure Proportioning
Max. Flow RateBinary High Pressure Gradient Pump: Nano ProFlow Flow Meter: 1500 nL/min; Capillary Flow Meter: 15 μL/min; Micro Flow Meter: 50 μL/min;; Ternary Micro Pump: 2500 μL/min
TypeBinary for Column, Ternary for Loading
Gradient Delay Volume<25 nL (binary)
Pressure RangeBinary High Pressure Gradient Pump: Nano ProFlow Flow Meter: 0 to 900 bar; Capillary Flow Meter: 0 to 800 bar; Micro Flow Meter: 0 to 800 bar;; Ternary Micro Pump: 0 to 620 bar
Max. Pressure (Valves)up to 12,500 psi
No. of Solvents2 (column) and 3 (loading)
Proportioning Accuracy±1.0% of full scale/<0.3% SD
Wetted PartsMP35 N, titanium, PEEK, UHMW-PE, PTFE, FEP, sapphire, ZrO2, Al2O3, fused silica, SST, PCTFE, perfluoro elastomer (FFKM)
Eluent Bottles2 x 100 mL and 3 x 1000 mL
Switching ValvesUp to Two Valves: 6-port 2-position; 10-port, 2-position
Leak SensorYes
Piston Seal WashYes
Safety FeaturesLeak Sensor
Vacuum DegasserExternal/Optional
DescriptionNCS-3500RS Nano LC Pump with ProFlow Flow Meter and Column Compartment
Column Temperature RangeAmbient to 75.0°C
Dimensions (HWD)14.2 x 16.5 x 20 in. (36 x 42 x 51 cm)
Available (Optional) Flow MetersCapillary flow meter: 0-15 µL/min (Recommended 0.5-10 µL/min)<br /> Micro flow meter: 0-50 µL/min (Recommended 5-50 µL/min)
Unit SizeEach
Catalog Number
Unit Size
Each
Description
NCS-3500RS Nano LC Pump with Capillary Flow Meter and Column Compartment
Price (USD)
Full specifications
Min. Flow RateBinary High Pressure Gradient Pump: Nano ProFlow Flow Meter: 0 nL/min; Capillary Flow Meter: 0 μL/min; Micro Flow Meter: 0 μL/min;; Ternary Micro Pump: 0 μL/min
Operating Principlebinary high pressure gradient mixing pump (column) and ternary low pressure gradient mixing (trap-column loading)
Solvent DegassingExternal/Optional
Biocompatible VersionYes
Gradient FormationHigh-Pressure Proportioning
Max. Flow RateBinary High Pressure Gradient Pump: Nano ProFlow Flow Meter: 1500 nL/min; Capillary Flow Meter: 15 μL/min; Micro Flow Meter: 50 μL/min;; Ternary Micro Pump: 2500 μL/min
TypeBinary for Column, Ternary for Loading
Gradient Delay Volume<25 nL (binary)
Pressure RangeBinary High Pressure Gradient Pump: Nano ProFlow Flow Meter: 0 to 900 bar; Capillary Flow Meter: 0 to 800 bar; Micro Flow Meter: 0 to 800 bar;; Ternary Micro Pump: 0 to 620 bar
Max. Pressure (Valves)up to 12,500 psi
No. of Solvents2 (column) and 3 (loading)
Proportioning Accuracy±1.0% of full scale/<0.3% SD
Wetted PartsMP35 N, titanium, PEEK, UHMW-PE, PTFE, FEP, sapphire, ZrO2, Al2O3, fused silica, SST, PCTFE, perfluoro elastomer (FFKM)
Eluent Bottles2 x 100 mL and 3 x 1000 mL
Switching ValvesUp to Two Valves: 6-port 2-position; 10-port, 2-position
Leak SensorYes
Piston Seal WashYes
Safety FeaturesLeak Sensor
Vacuum DegasserExternal/Optional
DescriptionNCS-3500RS Nano LC Pump with Capillary Flow Meter and Column Compartment
Column Temperature RangeAmbient to 75.0°C
Dimensions (HWD)14.2 x 16.5 x 20 in. (36 x 42 x 51 cm)
Available (Optional) Flow MetersNano ProFlow meter with ProFlow technology:<br /> 0-1500 nL/min (Recommended 50-1500 nL/min)<br /> <br /> Micro flow meter:<br /> 0-50 µL/min (Recommended 5-50 µL/min)
Unit SizeEach
Showing 2 of 2
Catalog NumberSpecificationsUnit SizeDescriptionPrice (USD)
5041.0010AFull specifications
EachNCS-3500RS Nano LC Pump with ProFlow Flow Meter and Column CompartmentRequest A Quote
Min. Flow RateBinary High Pressure Gradient Pump: Nano ProFlow Flow Meter: 0 nL/min; Capillary Flow Meter: 0 μL/min; Micro Flow Meter: 0 μL/min;; Ternary Micro Pump: 0 μL/min
Operating Principlebinary high pressure gradient mixing pump (column) and ternary low pressure gradient mixing (trap-column loading)
Solvent DegassingExternal/Optional
Biocompatible VersionYes
Gradient FormationHigh-Pressure Proportioning
Max. Flow RateBinary High Pressure Gradient Pump: Nano ProFlow Flow Meter: 1500 nL/min; Capillary Flow Meter: 15 μL/min; Micro Flow Meter: 50 μL/min;; Ternary Micro Pump: 2500 μL/min
TypeBinary for Column, Ternary for Loading
Gradient Delay Volume<25 nL (binary)
Pressure RangeBinary High Pressure Gradient Pump: Nano ProFlow Flow Meter: 0 to 900 bar; Capillary Flow Meter: 0 to 800 bar; Micro Flow Meter: 0 to 800 bar;; Ternary Micro Pump: 0 to 620 bar
Max. Pressure (Valves)up to 12,500 psi
No. of Solvents2 (column) and 3 (loading)
Proportioning Accuracy±1.0% of full scale/<0.3% SD
Wetted PartsMP35 N, titanium, PEEK, UHMW-PE, PTFE, FEP, sapphire, ZrO2, Al2O3, fused silica, SST, PCTFE, perfluoro elastomer (FFKM)
Eluent Bottles2 x 100 mL and 3 x 1000 mL
Switching ValvesUp to Two Valves: 6-port 2-position; 10-port, 2-position
Leak SensorYes
Piston Seal WashYes
Safety FeaturesLeak Sensor
Vacuum DegasserExternal/Optional
DescriptionNCS-3500RS Nano LC Pump with ProFlow Flow Meter and Column Compartment
Column Temperature RangeAmbient to 75.0°C
Dimensions (HWD)14.2 x 16.5 x 20 in. (36 x 42 x 51 cm)
Available (Optional) Flow MetersCapillary flow meter: 0-15 µL/min (Recommended 0.5-10 µL/min)<br /> Micro flow meter: 0-50 µL/min (Recommended 5-50 µL/min)
Unit SizeEach
5041.0020Full specifications
EachNCS-3500RS Nano LC Pump with Capillary Flow Meter and Column CompartmentRequest A Quote
Min. Flow RateBinary High Pressure Gradient Pump: Nano ProFlow Flow Meter: 0 nL/min; Capillary Flow Meter: 0 μL/min; Micro Flow Meter: 0 μL/min;; Ternary Micro Pump: 0 μL/min
Operating Principlebinary high pressure gradient mixing pump (column) and ternary low pressure gradient mixing (trap-column loading)
Solvent DegassingExternal/Optional
Biocompatible VersionYes
Gradient FormationHigh-Pressure Proportioning
Max. Flow RateBinary High Pressure Gradient Pump: Nano ProFlow Flow Meter: 1500 nL/min; Capillary Flow Meter: 15 μL/min; Micro Flow Meter: 50 μL/min;; Ternary Micro Pump: 2500 μL/min
TypeBinary for Column, Ternary for Loading
Gradient Delay Volume<25 nL (binary)
Pressure RangeBinary High Pressure Gradient Pump: Nano ProFlow Flow Meter: 0 to 900 bar; Capillary Flow Meter: 0 to 800 bar; Micro Flow Meter: 0 to 800 bar;; Ternary Micro Pump: 0 to 620 bar
Max. Pressure (Valves)up to 12,500 psi
No. of Solvents2 (column) and 3 (loading)
Proportioning Accuracy±1.0% of full scale/<0.3% SD
Wetted PartsMP35 N, titanium, PEEK, UHMW-PE, PTFE, FEP, sapphire, ZrO2, Al2O3, fused silica, SST, PCTFE, perfluoro elastomer (FFKM)
Eluent Bottles2 x 100 mL and 3 x 1000 mL
Switching ValvesUp to Two Valves: 6-port 2-position; 10-port, 2-position
Leak SensorYes
Piston Seal WashYes
Safety FeaturesLeak Sensor
Vacuum DegasserExternal/Optional
DescriptionNCS-3500RS Nano LC Pump with Capillary Flow Meter and Column Compartment
Column Temperature RangeAmbient to 75.0°C
Dimensions (HWD)14.2 x 16.5 x 20 in. (36 x 42 x 51 cm)
Available (Optional) Flow MetersNano ProFlow meter with ProFlow technology:<br /> 0-1500 nL/min (Recommended 50-1500 nL/min)<br /> <br /> Micro flow meter:<br /> 0-50 µL/min (Recommended 5-50 µL/min)
Unit SizeEach
Showing 2 of 2
Easy flow rate switching

This pump is integrated with a loading pump and column compartment in a single module, pre-configured for:

  • Nano-flow (5041.0010A, NCS-3500RS Nano/Cap ProFlow System)
  • Capillary-flow (5041.0020, NCS-3500RS Capillary-flow Nano/Cap-System)
  • Or as a standalone pump (5041.0030A, NCP-3200RS Nano/Cap ProFlow Pump).

Nano, capillary or microflow ranges are selected by simply exchanging the dedicated flow meter

The right set of advantages
  • ProFlow technology for nano-flow applications provides the highest level of retention time precision
  • Capillary flow meter enables high-sensitivity with high-throughput analyses for reliable routine assays
  • Continuous-flow high pressure gradient pump provides uninterrupted gradient delivery permitting ultra-long gradients
  • An additional micro flow pump provides uninterrupted continuous flow through the trap and analytical column, even during sample loading, making sample pre-concentration and two dimensional workflows possible
  • UHPLC pressure capability of up to 860 bar for high performance separations
Added flexibility

This single system can accommodate your present and future needs. The unique combination of a UHPLC binary nano/cap/micro pump with a microloading pump adapts seamlessly to both standard and complex nano and capillary UHPLC workflows.

Improved repeatability

The thermostatted column compartment with up to two high-pressure nano switching valves can be heated up to 75°C to increase chromatographic speed and repeatability. The snap-in valves allow toolfree removal and can be pulled forward for easily making fluidic connections. The column compartment accommodates coiled nano columns up to one meter in length.

Seamless LC-MS integration
  • Dedicated connection kits and LC-MS interfaces for straightforward hardware integration
  • Seamless software integration with Thermo Scientific mass spectrometers via Thermo Scientific™ SII and single-point intelligent control with the easy-to-use Thermo Scientific™ Chromeleon™ Chromatography Data System
Features
  • Continuous direct flow provides uninterrupted runs as long as eluent is available
  • Accurate flow delivery from 50 nL/min up to 50µL/min. at pressures up to 86MPa (860 bar)
  • Small gradient delay volume of only 25nL
  • Thermo Scientific™ nanoViper™ Fingertight Fitting system provides virtually zero dead volume connections

Figures

Frequently asked questions (FAQs)

Citations & References