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JAM2 Recombinant Mouse Protein, hIgG1-Fc Tag

Catalog number: 50464M02H25

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Description

Junction Adhesion Molecule 2 (JAM2), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in performing cell based assays. It can also be used as a protein standard, or in other research applications.

This recombinant protein was expressed from a DNA sequence encoding the mouse JAM2 (NP_076333.3) extracellular domain (Met 1-Asn 236) fused to the Fc region of human IgG1 at the C-terminus.

Activity: Measured by the ability of the immobilized protein to support the adhesion of Jurkat human leukemic T cells. When 8 x 104 cells/well are added to JAM2 coated plates (0.2 µg/ml and 100 µl/well), approximately 40-55% will adhere specifically after 60 minutes at 37°C.

Formulation: Lyophilized in 40 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by Sino Biological Inc.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Protein Family: CD Proteins
Research Category: Other Research
Endotoxin Level: < 1 EU/µg
Expression: Mammalian
Form: Lyophilized
Gene Aliases: RP23-253E17.2, 1110002N23Rik, 2410030G21Rik, 2410167M24Rik, AU016127, JAM-2, JAM-B
Gene Symbol: JAM2
Molecular Weight: 50.3 kDa
Product Line: Sino Biological®
Product Size: 5 x 5 µg
Protein Name: junction adhesion molecule 2
Protein Subtype: Intercellular adhesion Molecule
Protein Tag or Fusion: IgG1 Fc Tag
Purity: ≥ 90% by SDS-PAGE
Species: Mouse
Validated Application: Assay Standard

Contents & storage

Includes 5 vial of lyophilized protein. Store at -20 °C.

Documents

Manuals & protocols