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KITL Recombinant Mouse Protein, His Tag

Catalog number: 50487M08B250

 Related applications: Protein Assays and Analysis

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Description

KIT ligand (KITL), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.

This recombinant protein was expressed from a DNA sequence encoding the mouse KITL (P20826-1) (Met 1-Ala 189) fused to a polyhistidine tag at the C-terminus.

Activity:
1. Measured by its binding ability in a functional ELISA . Immobilized mouse KITL at 2 µg/ml(100 µl/well) can bind mouse KIT with a linear ranger of 1.28-32 ng/ml.
2. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells (Kitamura,T. et al. 1989, J. Cell Physiol. 140:323) . The EC50 for this effect is typically 0.5-5 ng/ml.

Formulation: Lyophilized in 20 mM Tris, 500 mM NaCl, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.



This protein is manufactured by Sino Biological Inc.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Research Category: Cell Cycle & Proliferation, Drug Development, Oncology, Signal Transduction, Stem Cell Research
Endotoxin Level: < 1 EU/µg
Expression: Insect
Form: Lyophilized
Gene Aliases: Clo, Con, Gb, Kitlg, Mgf, SCF, SF, SLF, Sl, Steel, c ontrasted
Gene Symbol: KITL
Molecular Weight: 19.7 kDa
Product Line: Sino Biological®
Product Size: 5 x 50 µg
Protein Family: Growth Factors & Receptors
Protein Form: Cell Surface Protein, Recombinant, Truncated
Protein Name: KIT ligand
Protein Subtype: Other Growth Factors
Protein Tag or Fusion: His Tag
Purity: ≥ 90% by SDS-PAGE
Species: Mouse
Validated Application: Assay Standard

Contents & storage

Includes 5 vial of lyophilized protein. Store at -20 °C.

Documents

Manuals & protocols

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