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KITL Recombinant Mouse Protein, His Tag

Catalog number: 50487M08B50

 Related applications: Protein Assays and Analysis

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KIT ligand (KITL), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.

This recombinant protein was expressed from a DNA sequence encoding the mouse KITL (P20826-1) (Met 1-Ala 189) fused to a polyhistidine tag at the C-terminus.

1. Measured by its binding ability in a functional ELISA . Immobilized mouse KITL at 2 µg/ml(100 µl/well) can bind mouse KIT with a linear ranger of 1.28-32 ng/ml.
2. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells (Kitamura,T. et al. 1989, J. Cell Physiol. 140:323) . The EC50 for this effect is typically 0.5-5 ng/ml.

Formulation: Lyophilized in 20 mM Tris, 500 mM NaCl, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by Sino Biological Inc.

For Research Use Only. Not for use in diagnostic procedures.


Form: Lyophilized
Purity: ≥ 90% by SDS-PAGE
Species: Mouse
Expression: Insect
Gene Symbol: KITL
Gene Aliases: Clo, Con, Gb, Kitlg, Mgf, SCF, SF, SLF, Sl, Steel, c ontrasted
Product Size: 50 µg
Protein Form: Truncated, Recombinant, Cell Surface Protein
Protein Family: Growth Factors & Receptors
Endotoxin Level: < 1 EU/µg
Protein Subtype: Other Growth Factors
Molecular Weight: 19.7 kDa
Research Category: Signal Transduction, Cell Cycle & Proliferation, Stem Cell Research, Drug Development, Oncology
Protein Tag or Fusion: His Tag
Validated Application: Assay Standard

Contents & storage

Includes 1 vial of lyophilized protein. Store at -20 °C.


Manuals & protocols

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