Thermo Scientific™

UltiMate™ 3000 Fluorescence Detectors

Related applications:

Industrial Chromatography

Develop highly selective methods for trace analytes or novel molecules with sensitivity three to six orders of magnitude greater than UV detection. The Thermo Scientific™ Dionex™ UltiMate™ 3000 Fluorescence Detectors offer four channel acquisition at up to 200Hz so you can optimize quantification on the most sensitive channel and single-channel detection at up to 100Hz. 2D or 3D synchro, excitation, emission scans, zero order mode, or multichannel acquisition offer flexibility for method development or routine sample characterization. A variable emission filter allows real-time, compound-related sensitivity optimization.
Back to top
Recommended Use

The Thermo Scientific™ UltiMate 3000 Rapid Separation Fluorescence Detector FLD-3400 is recommended for method development labs that need flexibility in detecting novel compounds that don’t absorb in the UV-vis region of the spectrum. It is ideal for the analysis of carbohydrates, glycans, and polyaromatic hydrocarbons (PAHs) in combination with any Thermo Scientific™ Dionex™ UltiMate 3000 system. The ability to cleave and derivitize glycans from intact proteins or monoclonal antibodies eliminates interferences from coeluting matrix peptides.

Sensitive Fluorescence Performance

  • Data rates at up to 200Hz detect the narrowest peaks in UHPLC separations.
  • Maximum stray light suppression provides excellent sensitivity.
  • Low limits of detection are possible with a Raman S/N: >550 ASTM (>2100 using dark signal as noise reference).
  • Active flow cell temperature control ensures stable fluorophore activity independent of ambient temperature.
  • Long-life xenon flash lamp delivers the highest sensitivity and long-term operation.
  • Optional second photomultiplier (PMT) for Dual-PMT operation extends the wavelength range up to 900nm without sacrificing sensitivity in the standard wavelength range.

Easy-to-Use Fluorescence Detector

  • Large front panel display allows easy monitoring of detector status.
  • Flow cell syringe injection kit enables cost-effective off-line measurements.
  • Flow cell optimized for trace analysis with narrow-bore UHPLC and conventional HPLC columns.
  • Automate qualification monitoring and tracking of flow cells for regulatory compliance.
  • Thermo Scientific™ Dionex™ Chromeleon™ Chromatography Data System software offers performance optimization and instrument wellness monitoring for ease of use and maximum uptime.