Opti-MEM™ I Reduced Serum Medium, GlutaMAX™ Supplement

인용 및 참조 문헌 (1)

Gibco™

Opti-MEM™ I Reduced Serum Medium, GlutaMAX™ Supplement

Opti-MEM™ Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation자세히 알아보기

카탈로그 번호	수량
51985026 51985-026으로도 사용됨	500 mL

카탈로그 번호 51985026

51985-026으로도 사용됨

제품 가격(KRW)

500 mL

Customize this product

Opti-MEM™ Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change to growth rate or morphology. Opti-MEM™ is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM™ can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. We offer a variety of Gibco™ Opti-MEM™ modifications for a range of cell culture applications.

This Opti-MEM™ is modified as follows:

• GlutaMAX™

• Phenol Red

The complete formulation is confidential. For more information, please contact Technical Services.

Gibco™ Opti-MEM™ is a unique media that contains insulin, transferrin, hypoxanthine, thymidine, and trace elements. These additional components allow for a reduction in serum supplementation by at least 50%. Gibco™ Opti-MEM™ with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format.

Gibco™ Opti-MEM™ uses a sodium bicarbonate buffer system (2.4 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.

세포 유형Mammalian Cells

농도1 X

배양 환경CO₂

제조 품질cGMP-compliant under the ISO 13485 standard

삼투압280 - 320 mOsm/kg

제품라인Gibco, Opti-MEM

제품 유형Reduced-Serum Medium

수량500 mL

유통 기한18 Months

배송 조건Room Temperature

분류Animal Origin

Culture TypeMammalian Cell Culture

형태Liquid

멸균Sterile-filtered

첨가제 포함GlutaMAX, HEPES, Phenol Red, Sodium Pyruvate, Sodium Bicarbonate

pH7 to 7.4

Unit SizeEach

구성 및 보관

Storage conditions: 2-8° C. Protect from light.
Shipping conditions: Ambient.
Shelf life: 18 months from date of manufacture.

자주 묻는 질문(FAQ)

Is there any other difference between Opti-MEM I Reduced Serum Medium and Opti-MEM I Reduced Serum Medium, no phenol red besides the presence/absence of phenol red?

The formulation of Opti-MEM I Reduced Serum Medium and that of Opti-MEM I Reduced Serum Medium, no phenol red is the same except for the presence of phenol red in the former medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Where can I find the osmolality for my lot of Opti-MEM I Reduced Serum Medium?

We do provide osmolality information on the certificate of analysis. All lots of Opti-MEM I Reduced Serum Medium will meet the osmolality specification of ≥280 to ≤320 mOsm/kg.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I aliquot and freeze Opti-MEM I Reduced Serum Medium to conserve it longer? If so, for how long can it be stored?

Sorry, we don't have data to show that frozen Opti-MEM I Reduced Serum Medium has a longer shelf life. Actually, we recommend storing this medium at 2-8 degrees C in the dark. We do not recommend freezing the medium because it may precipitate.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I noticed that Opti-MEM I Reduced Serum Medium does not contain any serum. Why do you then refer to it as "Reduced Serum Medium"?

Opti-MEM I Reduced Serum Medium is a serum-free medium. The reason why it is called “reduced-serum medium” is because you only need to supplement this medium with 1-5% FBS instead of the standard 10% FBS.

Opti-MEM I Reduced Serum Medium can be used as is (without serum) for transfection of cells. However, for long-term cell culture, you need to add serum to the medium to keep cells healthy.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Will depletion, absence, or breakdown of essential growth-promoting components such as glutamine or growth factors reduce the growth rate of my culture?

Yes. If you suspect that this is the case, remove the medium and add fresh medium. Alternatively, you can supplement medium with growth-promoting components. It is also possible to substitute GlutaMax I or II for glutamine in the medium to prevent glutamine exhaustion.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Opti-MEM™ I Reduced Serum Medium, GlutaMAX™ Supplement, 500 mL FAQs

인용 및 참조 문헌 (1)

인용 및 참조 문헌

Abstract

A solution for highly efficient electroporation of primary cytotoxic T lymphocytes.

Authors:Alawar N,Schirra C,Hohmann M,Becherer U

Journal:BMC biotechnology

PubMed ID:38532411

BACKGROUND: Cytotoxic T lymphocytes (CTLs) are central players in the adaptive immune response. Their functional characterization and clinical research depend on efficient and reliable transfection. Although various methods have been utilized, electroporation remains the preferred technique for transient gene over-expression. However, the efficiency of electroporation is reduced for human and ... More