Opti-MEM® I Reduced Serum Medium, GlutaMAX™ Supplement This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number: 51985091

Gibco™  Related applications: Mammalian Cell Culture | Transfection

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Description

Opti-MEM® I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change in growth rate or morphology. Opti-MEM® I medium is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM® I medium can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. We offer a variety of Opti-MEM® I modifications for a range of cell culture applications.

This Opti-MEM® I is modified as follows:
With
• L-glutamine
• Phenol Red

The complete formulation is confidential. For more information, please contact Technical Services.

Using Opti-MEM® I Medium
Opti-MEM® I Reduced-Serum Medium is a unique medium that contains insulin, transferrin, hypoxanthine, thymidine, and trace elements. These additional components allow for a reduction in serum supplementation by at least 50%. Opti-MEM® I medium uses a sodium bicarbonate buffer system (2.4 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

cGMP manufacturing and quality system
Opti-MEM® I is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Opti-MEM® I product made in our Scotland facility (51985-042). This facility is also registered with the FDA as a medical device manufacturer and certified to the ISO 13485 standard.

“This reagent is classified as a medical device in several countries. If it is not regulated as a medical device in specific region, it should be used for laboratory use only.”

Specifications

Glutamine: GlutaMAX™-I
Phenol Red Indicator: Phenol Red
Form: Liquid
Product Size: 10 x 500 mL
Volume (Metric): 500 ml
Green Features: Fewer resources used, Less waste
Shipping Condition: Room Temperature

Contents & storage

Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 18 months from date of manufacture

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Manuals & protocols

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