DyLight™ 488 Microscale Antibody Labeling Kit

Catalog number: 53025

Thermo Scientific™  Related applications: Protein Labeling & Crosslinking

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The Thermo Scientific DyLight 488 Microscale Antibody Labeling Kit contains an NHS ester-activated derivative of high-performance DyLight 488 for fluorescent labeling of antibodies and other proteins to be used as molecular probes for cellular imaging and other fluorescence detection methods. The microscale kit contains all of the necessary components to perform five separate labeling reactions using 100 µg of IgG.

DyLight 488 has high fluorescence intensity over a broad pH range (pH 4-9) and is more photostable than Cy2™, Alexa™ Fluor 488, FITC and LI-COR™ dyes in many applications. The high water solubility of DyLight Fluors allows a high dye-to-protein ratio to be achieved without causing precipitation of the conjugates. DyLight 488 Amine-Reactive Dye is also available as a stand-alone reagent.

Features of the DyLight 488 NHS Ester:

High performance— DyLight 488 is comparable to Alexa Fluor 488 and brighter than FITC and Cy2
Specific— NHS ester-activated dye labels proteins and other molecules at primary amines (-NH2)
Convenient kit sizes— standard and microscale sizes are offered to match your experimental needs
Optimized procedure— following the standard protocol results in antibodies with excellent dye:protein ratios and recovery rates for optimum activity and fluorescence labeling

• Primary antibody labeling for immunofluorescence microscopy, immunohistochemistry (IHC), Western blotting or ELISA assay
• Target protein labeling for in vitro and in vivo fluorescent detection strategies

DyLight 488 Amine-Reactive Dye is activated with an N-hydroxysuccinimide (NHS) ester moiety to react with exposed N-terminal α-amino groups or the ε-amino groups of lysine residues to form stable amide bonds. Learn more about NHS ester chemistry.

Typical labeling reactions require the dye to first be dissolved in anhydrous dimethyl formamide (DMF) or another suitable organic solvent before adding a specific molar amount of dye to an amine-free buffer containing the protein to be labeled. However, the high solubility of DyLight Fluors permits protein solutions to be added directly to specific amounts of the labeling reagent. This feature allows DyLight 488 Amine-Reactive Dye to be provided in multiple formats with flexible protocols to achieve efficient degrees of labeling.

We also offer Standard and Microscale DyLight 488 Antibody Labeling Kits for fast and efficient fluorescent labeling of antibodies for use in fluorescence methods. The standard size kit contains all necessary components to perform three separate labeling reactions using 1 mg of IgG or similar quantities of other proteins. The microscale kit contains all of the necessary components to perform five separate labeling reactions using 100 µg of IgG. Both kit sizes include the Amine-Reactive DyLight 488 NHS-ester in convenient single-use vials as well as purification resin and spin columns for the preparation of ready-to-use conjugate.

Related Products
DyLight™ 488 NHS Ester
DyLight™ 488 Antibody Labeling Kit
For Research Use Only. Not for use in diagnostic procedures.


Label or Dye: DyLight™ 488
Product Size: 5 reactions
Labeling Scale: 100 µg
Labeling Target: Proteins (General), Antibodies (General)
Excitation⁄Emission (nm): 493⁄518

Contents & storage

Sufficient For: Labeling and purifying 5 x 100 µg (1 mg/mL) of IgG or other proteins
• DyLight Fluor NHS Ester, 5 x 15 µg vials
• Borate Buffer (0.67M), 1 mL
• Purification Resin, 5 mL
• Microcentrifuge Spin Columns, 5 columns
• Microcentrifuge Collection Tubes, 10 tubes

Store the DyLight NHS Ester at -20°C. Store all other kit components at 4°C.


Manuals & protocols