Thermo Scientific™

Pierce™ FITC Antibody Labeling Kit

Catalog number: 53027
Thermo Scientific™

Pierce™ FITC Antibody Labeling Kit

Catalog number: 53027
Catalog Number
Unit Size
3 reactions
Price (USD)
Price: 436.00
Online price: 412.65
Your price:
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
530273 reactions
Price: 436.00
Online price: 412.65
Your price:
Product Overview
Citations & References
Additional Information
The Thermo Scientific FITC Antibody Labeling Kit is a multi-component kit containing a high-performance derivative of fluorescein dye, activated for easy and reliable labeling of antibodies, proteins and other molecules for use as fluorescent probes.

Features of the kit:

Easy —convenient kit with FITC to label and purify antibody in about one hour
Amine-specific labeling—the isothiocyanate variety of fluorescein efficiently labels antibodies and other purified proteins at primary amines (lysine side chains)
Optimized kit procedure—following the standard protocol results in antibodies with excellent dye:protein ratios for optimum activity and fluorescence
Single-use fluors—no need to weigh tiny amounts of powder; kits contain single-use vials of reagent
Efficient purification—kits include purification resin and easy-to-use spin columns, ensuring rapid and efficient removal of non-reacted dye and excellent protein recovery

Properties of FITC:

• Alternative names: 5/6-FITC
• Chemical name: 5(6)-fluorescein isothiocyanate mixed isomer
• Molecular weight: 389.2
• Excitation source: 488 nm spectral line, argon-ion laser
• Excitation wavelength: 494 nm
• Emission wavelength: 518 nm
• Extinction coefficient: > 70,000 M-1cm-1
• CAS #: 27072-45-3
• Purity: > 95% by HPLC
• Solubility: Soluble in aqueous buffers at pH > 6
• Reactive groups: Isothiocyanate, reacts with primary amines at pH 7.0 to 9.0

• Label antibodies for use as immunofluorescent probes
• Label oligonucleotides for hybridization probes
• Detect proteins in gels and on Western blots

Amine-reactive Fluorescein Dyes
FITC is the base fluorescein molecule functionalized with an isothiocyanate reactive group (—N=C=S) at one of two hydrogen atoms on the bottom ring of the structure. This derivative is reactive towards primary amine groups on proteins, peptides and other biomolecules. NHS-fluorescein is activated with the N-hydroxy-succinimidyl-ester (NHS ester) functional group. Compared to FITC, the NHS-ester deriviative has greater specificity toward primary amines in the presence of other nucleophiles and results in a more stable linkage following labeling. Pierce Amine-reactive Fluorescein Dyes are mixtures of isomers with reactive groups attached at the 5- and 6-positions of the bottom ring. The properties of these isomers are indistinguishable in terms of excitation and emission spectra, and for protein applications there is no need to isolate a specific isomer.

Related Products
NHS-Fluorescein (5/6-carboxyfluorescein succinimidyl ester), mixed isomer
FITC (5/6-fluorescein isothiocyanate), mixed isomer
Pierce™ NHS-Fluorescein Antibody Labeling Kit
For Research Use Only. Not for use in diagnostic procedures.


Pierce FITC Antibody Labeling Kit
Product Type
Labeling Kit
1 Kit
Sufficient For
Labeling and purifying 3 x 1 mg (2 mg/mL) of IgG or other proteins
Labeling Scale
0.5 to 1 mg
Product Line
Aqueous Buffer
Label or Dye
FITC (Fluorescein)
Labeling Target
Antibodies, Proteins

Contents & Storage

  • FITC, 3 x 50 μg vials
  • Borate Buffer, 1 mL
  • Purification Resin, 5 mL
  • Spin Columns, 6 each
  • Collection Tubes, 12 each


Documents & Downloads


    Frequently asked questions (FAQs)

    Citations & References

    Search citations by name, author, journal title or abstract text