Thermo Scientific™

Pierce™ NHS-Rhodamine Antibody Labeling Kit

Catalog number:  53031

 Related applications:

Protein Labeling & Crosslinking

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Thermo Scientific NHS-Rhodamine is a high-performance derivative of rhodamine dye, activated for easy and reliable labeling of antibodies, proteins and other molecules for use as fluorescent probes. Use our NHS-Rhodamine Antibody Labeling Kit to label and purify antibodies in about one hour.

Features of NHS-Rhodamine :

Amine-specific labeling—NHS-ester varieties of rhodamine efficiently label antibodies and other purified proteins at primary amines (lysine side chains)
Optimized procedure—following the standard protocol results in antibodies with excellent dye:protein ratios for optimum activity and fluorescence
Single-use fluors—no need to weigh tiny amounts powder; kit contains single-use vials of reagent
Efficient purification—kit includes purification resin and easy-to-use spin columns, ensuring rapid and efficient removal of non-reacted dye and excellent protein recovery

NHS Rhodamine is an amine-reactive derivative of rhodamine dye that has wide-ranging application as antibody and other probe labels for use in fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as western blotting and ELISA.

• Label antibodies for use as immunofluorescent probes
• Label oligonucleotides for hybridization probes
• Detect proteins in gels and on western blots

Properties of Rhodamine Dyes:
Thermo Scientific Pierce Rhodamine Dyes are mixtures of isomers with reactive groups attached at the 5- and 6-positions of the bottom ring. The properties of these isomers are indistinguishable in terms of excitation and emission spectra, and for protein applications there is no need to isolate a specific isomer.

NHS-Rhodamine is activated with the N-hydroxy-succinimidyl-ester (NHS ester) functional group. Compared to TRITC, the NHS-ester deriviative has greater specificity toward primary amines in the presence of other nucleophiles and results in a more stable linkage following labeling.

Application Data:

Related Products
NHS-Rhodamine (5/6-carboxy-tetramethyl-rhodamine succinimidyl ester), mixed isomer
TRITC (5/6-tetramethyl-rhodamine isothiocyanate), mixed isomer
For Research Use Only. Not for use in diagnostic procedures.


Excitation⁄Emission (nm): 552⁄575
Label or Dye: Rhodamine Isomers
Labeling Scale: 0.5-1 mg
Labeling Target: Antibodies (General), Proteins (General)
Product Line: Pierce™
Product Size: 3 reactions

Contents & storage

Sufficient For: Labeling and purifying 3 x 1 mg (2 mg/mL) of IgG or other proteins
• NHS Rhodamine, 3 x 35 µg vials
• Borate Buffer, 1 mL
• Purification Resin, 5 mL
• Spin Columns, 6 each
• Collection Tubes, 12 each

Store NHS-Rhodamine at -20°C. Store all other components at 4°C.


Manuals & protocols