SeCore® B Locus Sequencing Kit (RUO)

Catalog number: 5310925

Invitrogen™  Related applications: Diagnostic Testing

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The Class I SeCore sequencing strategy begins with a multiplex PCR. Two fragments are amplified; one amplicon encompassing exon 2 and 3 and the other encompassing exon 4. The sequencing mixes are designed for bi-directional sequencing of each exon. The B Locus kit includes two amplification mixes, each specific to a set of alleles. Heterozygous sample alleles are often amplified by different mixes and therefore are separated by this initial PCR. The resulting typing data for each separated allele is a greatly simplified homozygous electropherogram, eliminating the challenge of interpreting polymorphic positions. This strategy reduces the cis/trans ambiguities for B locus by 45%. The Class I Z GSSP Kits are designed to resolve ambiguous allele combinations encountered with the SeCore A, B and C Locus Sequencing Kits. Additional amplification is not necessary; the original amplicon serves as the GSSP template. To identify the GSSP Kit that will resolve your ambiguity click on the link "SeCore GSSP Selection Table".

Contents & storage

Store in freezer (-5 to -30°C).


Manuals & protocols