SeCore® B Locus Sequencing Kit, Single Amplification System

Catalog number: 5311025D

 Related applications: Diagnostic Testing

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The Class I SeCore sequencing strategy begins with a multiplex PCR. Two fragments are amplified; one amplicon encompassing exon 2 and 3 and the other encompassing exon 4. The sequencing mixes are designed for bi-directional sequencing of each exon.

The B Locus kit Group Specific Set includes two amplification mixes, each specific to a set of alleles. Heterozygous sample alleles are often amplified by different mixes and therefore are separated by this initial PCR. The resulting typing data for each separated allele is a greatly simplified homozygous electropherogram, eliminating the challenge of interpreting polymorphic positions. This strategy reduces the cis/trans ambiguities for B locus by 45%.

The Class I Z GSSP Kits are designed to resolve ambiguous allele combinations encountered with the SeCore A, B and C Locus Sequencing Kits. Additional amplification is not necessary; the original amplicon serves as the GSSP template.

The SeCore® HLA Sequencing System is intended for the identification and definition of Class I and II Human Leukocyte Antigens (HLA) and is for use in HLA typing. The SeCore® HLA Sequencing System provides human histocompatibility information of HLA Class I (A, B, and C) and Class II (DPB1, DQB1, and DR) loci using genomic DNA isolated from whole blood specimens. HLA typing using the SeCore® Sequencing Kits must be performed in the presence of an HLA Lab Director, Technical Supervisor and/or general Supervisor following accepted laboratory accreditation standards (ASHI). These products are for professional use only. This test must not be used as the sole basis for making clinical decisions. For the intended use of individual system components, refer to the documentation provided with the component.

“This reagent is classified as a medical device in several countries. If it is not regulated as a medical device in specific region, it should be used for laboratory use only.”

Contents & storage

Store in freezer (-5 to -30°C).


Manuals & protocols