Pierce™ Streptavidin Plus UltraLink™ Resin
Pierce™ Streptavidin Plus UltraLink™ Resin
Thermo Scientific™

Pierce™ Streptavidin Plus UltraLink™ Resin

The Thermo Scientific™ Pierce™ High Capacity Streptavidin Chromatography Cartridges are convenient, ready-to-use devices for separating biotinylated molecules from non-biotinylated moleculesRead more
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Catalog NumberQuantitySufficient For
531162 mLBinding ≥/= 48 μg Biotin/mL Resin (≥/= 4 mg Biotinylated BSA/mL Resin)
531175 mLBinding ≥/= 48 μg Biotin/mL Resin (≥/= 4 mg Biotinylated BSA/mL Resin)
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Catalog number 53116
Price (USD)/ 2 mL
420.65
Online exclusive
468.00 
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Quantity:
2 mL
Sufficient For:
Binding ≥/= 48 μg Biotin/mL Resin (≥/= 4 mg Biotinylated BSA/mL Resin)
Request bulk or custom format
Price (USD)/ 2 mL
420.65
Online exclusive
468.00 
Save 47.35 (10%)
Add to cart
Pierce™ Streptavidin Plus UltraLink™ Resin
Catalog number53116
Price (USD)/ 2 mL
420.65
Online exclusive
468.00 
Save 47.35 (10%)
-
Add to cart
The Thermo Scientific™ Pierce™ High Capacity Streptavidin Chromatography Cartridges are convenient, ready-to-use devices for separating biotinylated molecules from non-biotinylated molecules and for purifying antigens using biotinylated antibodies. The chromatography cartridges contain a beaded resin of immobilized recombinant streptavidin protein that binds biotinylated proteins at greater than 10 mg/mL of beads. This corresponds to 2 to 3 times higher biotin-binding capacity than products from other suppliers and is ideal when a high amount of recovery is required.

These cartridges enable fast, easy, and reproducible chromatographic separations and are compatible with the major automated liquid-chromatography systems or manual syringe processing (see Table 1 for cartridge general properties). The cartridges attach directly to ÄKTA™ or FPLC systems without additional connectors. The included accessory pack readily adapts cartridges for use with Luer-Lok™ syringe fittings or 1/16' tubing. These cartridges can be regenerated multiple times for affinity purification.

Features of High Capacity Streptavidin Agarose:
Streptavidin—purified recombinant streptavidin protein (53 kDa, near-neutral pI); tetrameric with four biotin-binding site per molecule
Agarose resin—support is cross-linked 6% beaded agarose (CL-6B), the most popular resin for protein affinity purification methods
Inert and stable—streptavidin is immobilized by charge-free, leach-resistant covalent bonds, resulting in low nonspecific binding and enabling multiple uses without decline in yield
High capacity—this variety of beads has a dense load of immobilized streptavidin, providing a binding capacity greater than 10 mg of biotinylated BSA per mL of resin
Two formats—available in several bottle sizes of resin slurry and as packed 1 mL and 5 mL chromatography cartridges

Properties of cross-linked 6% beaded agarose (CL-6B):
• Support pH stability: 2 to 14 (short term); 3 to 13 (long term)
• Average particle size: 45 to 165 microns
• Exclusion limit: 10,000 to 4,000,000 daltons
• Maximum recommended flow rate: 4 mL/minute (1 mL column), 5 mL/min (5 mL column)
• Maximum linear velocity: 30 cm per hour
• Maximum pressure: 0.3 MPa, 43.5 psi or 3 bar

Pierce High Capacity Streptavidin Chromatography Cartridges consist of purified recombinant streptavidin covalently immobilized at a high density onto high-quality cross-linked 6% beaded agarose. Biotinylated antibodies, proteins, peptides, nucleic acids, and other molecules or interaction complexes can be captured, immunoprecipitated, removed, or purified from samples using this streptavidin resin.

Streptavidin is a tetrameric protein containing four biotin-binding sites similar to avidin. The native protein from Streptomyces avidinii is carbohydrate-free, has an acidic pI of 5.5 and a mass of 75 kDa. The recombinant protein used to manufacture Pierce streptavidin resins has a near-neutral isoelectric point and a mass of 53 kDa. Streptavidin generally has less nonspecific binding than avidin (from chicken egg white) because of the absence of carbohydrates.

For more information see: Chromatography Cartridges for Desalting and Affinity Purification
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Capacity (Metric)10 mg Biotinylated BSA Binding per mL Resin
Particle Size45 to 165 μm
Product LinePierce™, UltraLink™
Quantity2 mL
Protein FormRecombinant
TargetBiotin-Tagged Molecule
TypeResin
Stationary PhaseStreptavidin
Sufficient ForBinding ≥/= 48 μg Biotin/mL Resin (≥/= 4 mg Biotinylated BSA/mL Resin)
FormatBottle
Column TypeAffinity, UltraLink™ Resin
Unit Size2 mL
Contents & Storage
Upon receipt store at 4°C. Product is shipped at ambient temperature.