Clariom™ GO Screen Assay, Rat

The Clariom GO Screen research assay for rat samples is a 384-format microarray that provides a high-throughput, low-cost method to directly measure the expression of ∼20,000 genes. The design uses the Sprague-Dawley assembled genome. However, empirical probe selection on data across 452 samples where 50% were from Han-Wistar rats worked well in both strains of rat.

The content, developed in collaboration with pharma, includes probes derived from the Gene Ontology (GO) gene collection and is ideally suited for secondary compound library screening and hit profiling. The Clariom GO Screen Assay serves as a gene-level expression profiling tool and allows a fast, economical path to generate expression data for your research.

Clariom GO Screen data are compatible with our freely available Transcriptome Analysis Console (TAC) Software, which enables exploratory grouping analysis and identification of gene expression differences between clusters or groupings. The Clariom GO Screen Assay is the tool of choice for large testing efforts across ∼20,000 genes.

High volume at low cost

Through close collaboration with leaders in pharma research, the Clariom GO Screen Assay was designed to quickly assess changes in key genes and pathways in a high-throughput research testing environment. The assay enables higher throughput at a lower cost by incorporating direct lysis of samples, permitting researchers to bypass RNA purification for cell line applications. With a research workflow that is fully enabled by service provider partnerships and complimentary TAC Software, the Clariom GO Screen Assay helps researchers reach conclusions more rapidly.

Reliable gene expression analysis

The Clariom GO Screen Assay targets constitutively expressed exons that are least affected by alternative splicing, allowing an accurate summary of gene expression. Each gene is assayed with three unique probes selected for their specificity and uniqueness, as well as their performance across empirical data. By selecting the genes associated with the GO Consortium, the nomenclature and annotation provide a reliable source of material for functional assessment of the gene expression changes that have occurred. Inclusive of these ∼20,000 genes are virtually all the genes found in Wikipathways.org pathway, commercial panels for literature-associated 'biomarker' genes, immunology markers, and inflammation markers. The Clariom GO Screen research assay for rat samples helps provide the coverage you require, the analytical reproducibility you need, and the insights you want to act on your research discoveries.