Thermo Scientific™

CaptureSelect™ C-tagXL MiniChrom Column

Catalog number:  5943072001

Related applications:

Chromatography

Back to top

Description

CaptureSelect C-tagXL MiniChrom columns are designed for bench-scale resin screening, process development, and sample preparation. These pre-packed columns are ready to connect and have the following features:
• Dimensions of 1 mL (0.8 cm x 2 cm) and 5 mL (0.8 cm x 10 cm)
• Direct connection to standard chromatography systems
• Packed with CaptureSelect C-tagXL Affinity Matrix, offering high dynamic binding capacity and high resolution over a wide range of flow rates

The 1-mL columns can be used for quick screening of application feasibility and lab-scale purification on a convenient and easy-to-use pre-packed column. The 10-cm bed height of the 5-mL column allows initial process development on a bench scale.

CaptureSelect C-tagXL Affinity Matrix has a unique selectivity for a small 4-amino acid peptide tag (E-P-E-A, glutamic acid-proline-glutamic acid-alanine). CaptureSelect C-tagXL Affinity Matrix purifies the C-terminal tagged proteins with high affinity and selectivity, even in the presence of urea and guanidine HCl, from complex mixtures like cell culture harvests and periplasmic fractions in a one-step process. Mild elution conditions at neutral pH can be applied using magnesium chloride or propylene glycol, which ensures high-activity recoveries of pH-sensitive target proteins. The affinity resin recognizes the E-P-E-A tag sequence when fused either directly to the C-terminus of a protein or through a linker between the C-terminus and the E-P-E-A tag.

Features of this affinity matrix include:
• Mild elution, making it suitable for pH-sensitive proteins
• Binding of the tagged proteins under denaturing conditions (like dissolved inclusion bodies)
• Excellent scalability
• Non-animal-derived

View other biosimilars and recombinant protein affinity matrix formats ›

Free of animal components
CaptureSelect products contain affinity ligands created by a proprietary technology based on camelid-derived single-domain antibody fragments. The ligand is a 13-kDa single-domain fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced by the yeast Saccharomyces cerevisiae in a production process free of animal components.

Main characteristics:

Matrix: agarose-based, epoxide-activated
Average particle size: 65 ± 10 µm
Ligand: CaptureSelect C-tagXL affinity ligand
Ligand coupling method: epoxide coupling
Binding capacity: 400 nmol/mL resin depending on flow rate, column height, contact time
Elution conditions: Acidic: 20 mM citric acid or acetic acid, pH 3–4; 100 mM glycine buffer, pH 2–3. Neutral (pH 7.0–7.4): 20 mM Tris, 2.0 M MgCl2; 20 mM Tris, 1.0 M NaCl, 50% (v/v) propylene glycol; 20 mM Tris, 2 mM “S-E-P-E-A” peptide
Column dimensions: 1 mL (0.8 cm D x 2 cm L) and 5 mL (0.8 cm D x 10 cm L)
Flow characteristics: 75–150 cm/h (up to 2 bar)
Formulation buffer: 20%(v/v) ethanol

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Column Type: Prepacked
Product Line: CaptureSelect™
Shipping Condition: Wet Ice
Format: Chromatography Cartridge
Stationary Phase: C-tagXL ligand

Contents & storage

Store in refrigerator (2–8°C).