Thermo Scientific™

CaptureSelect™ KappaXL RoboColumn

Catalog number:  5943210050

 Related applications:

Chromatography

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Description

CaptureSelect™ KappaXL Affinity Matrix, available here in RoboColumn™ format, has been specifically designed for the purification of recombinant human Ig’s (including IgA, IgM, IgD, and IgE) containing a Kappa light chain and their Fab fragments. RoboColumns are small chromatography columns used for fully automated and parallel chromatographic separations on robotic liquid handling workstations.

Features of this affinity matrix include:
• Mild elution for Fab fragments and antibodies
• Human specific (e.g., no binding to bovine antibodies)
• Excellent scalability
• Non-animal-derived

CaptureSelect KappaXL Affinity Matrix purifies Ig’s, Fab, and Fab2 fragments directly from complex source materials in a single step with high purity and yield.

Free of animal components
CaptureSelect products are affinity ligands created by a proprietary technology based on camelid-derived single-domain antibody fragments. The ligand is a 13 kDa single-domain fragment comprising the 3 CDRs that form the antigen binding domain, efficiently produced by the yeast Saccharomyces cerevisiae in a production process free of animal components.

Characteristics:

Matrix: agarose-based, aldehyde-activated
Average particle size: 65 ± 10 µm
Ligand: CaptureSelect KappaXL affinity ligand
Coupling method: aldehyde coupling via NH2 residues of the ligand
Binding capacity: ~30 g IgG/L resin at 5-10 minutes contact time
Elution conditions: 20 mM citric acid, pH 3.5
Formulation buffer: 20%(v/v) ethanol

RoboColumn is a registered trademark of ATOLL GmbH.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Column Type: Affinity
Format: Agarose Bead
Immobilized Phase: CaptureSelect™ anti-Ig KappaXL
Product Line: CaptureSelect™
Product Size: 50 µL

Contents & storage

Store in refrigerator (2–8°C).

Documents

Manuals & protocols