Thermo Scientific™

CaptureSelect™ FcXL RoboColumn

Catalog number:  5943280050

 Related applications:

Chromatography

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Description

CaptureSelect™ FcXL Affinity Matrix, available here in RoboColumn™ format, has been specifically designed for the purification of recombinant human IgG, Fc fusion proteins, and plasma derived IgG. It recognizes the CH3 domain of all human IgG subclasses (IgG1, 2, 3, and 4). RoboColumns are small chromatography columns used for fully automated and parallel chromatographic separations on robotic liquid handling workstations.

Features of this affinity matrix include:
• Mild elution, making it suitable for Fc fusion proteins
• Human specific (e.g., no binding to bovine antibodies)
• Excellent scalability
• Non-animal-derived

CaptureSelect FcXL Affinity Matrix purifies IgGs and Fc fusion proteins directly from complex source materials in a single step with high purity and yield.

Free of animal components
CaptureSelect products are affinity ligands created by a proprietary technology based on camelid-derived single-domain antibody fragments. The ligand is a 13 kDa single-domain fragment comprising the 3 CDRs that form the antigen binding domain, efficiently produced by the yeast Saccharomyces cerevisiae in a production process free of animal components.

Characteristics:

Matrix: agarose-based, aldehyde-activated
Average particle size: 65 ± 10 µm
Ligand: CaptureSelect FcXL affinity ligand
Ligand coupling method: aldehyde coupling via NH2 residues of the ligand
Binding capacity: ~25 g IgG/L resin at 5-10 minutes contact time
Elution conditions: 20 mM citric acid or acetic acid, pH 3–4, or 20 mM sodium acetate, 1.0 M MgCl2, 40% (v/v) propylene glycol, pH 5–6
Formulation buffer: 20%(v/v) ethanol

RoboColumn is a registered trademark of ATOLL GmbH.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Column Type: Affinity
Format: Agarose Bead
Immobilized Phase: CaptureSelect™ anti-FcXL
Product Line: CaptureSelect™
Product Size: 50 µL

Contents & storage

Store in refrigerator (2–8°C).

Documents

Manuals & protocols