Thermo Scientific™

CaptureSelect™ CH1-XL RoboColumn

Catalog number:  5943462200

 Related applications:

Chromatography

| Clinical & Translational Research

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Description

CaptureSelect RoboColumns* are small chromatography columns used for fully automated and parallel chromatographic separations on robotic liquid handling workstations.

The CaptureSelect CH1-XL Affinity Matrix recognizes the CH1 domain of human IgG antibodies, thereby enabling purification of all Fab fragments, independent of the light-chain type (kappa or lambda) and without binding of over-expressed light chain and light-chain dimers. In addition, it can be used for the purification of human IgGs from all four subclasses (1, 2, 3, and 4).

Features of this affinity matrix include:
• Platform approach for purification of high quality and correctly assembled human Fab fragments
• No co-purification of over-expressed free light chains due to selectivity for the CH1 domain
• Elution possible at pH 4.0–4.5 with 50 mM acetate buffer
• Caustic cleaning with up to 50 mM NaOH
• Excellent scalability
• No cross-binding to bovine IgG, therefore presence of fetal bovine serum is not a problem
• Non-animal-derived

View other antibody affinity matrix formats

Free of animal components
CaptureSelect products are affinity ligands created by a proprietary technology based on camelid-derived single-domain antibody fragments. The ligand is a 13-kDa single-domain fragment comprising the three complementarity determining regions (CDRs) that form the antigen binding domain, efficiently produced by the yeast Saccharomyces cerevisiae in a production process free of animal components.

Characteristics:

Column size: 200 µL, 1 strip, 8 columns (8-row)
Column dimension: 5-mm diameter, 10-mm bed height
Matrix: agarose-based, epoxide-activated
Average particle size: 65 ± 10 µm
Ligand: CaptureSelect CH1-XL affinity ligand
Ligand coupling method: epoxide coupling
Binding capacity: >15 mg/mL human Fab fragment depending on flow rate, column height, contact time. For humanized Fabs, lower capacities may be observed.
Elution conditions: 100 mM glycine buffer, pH 3.0; 50 mM acetate buffer, pH 4.0–4.5
Formulation buffer: 20% (v/v) ethanol
Max operating pressure: 2 bar
Flow rate:16–150 cm/hr

*RoboColumn is a trademark of Repligen.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format: 8-well strips
Immobilized Phase: CaptureSelect CH1-XL affinity ligand
Product Line: CaptureSelect™
Product Size: 200 µL
Shipping Condition: Approved for shipment at Room Temperature or on Wet Ice

Contents & storage

Store in refrigerator (2–8°C).

Documents

Manuals & protocols