Thermo Scientific™

CaptureSelect™ CH1-XL RoboColumn

Catalog number: 5943462200
Thermo Scientific™

CaptureSelect™ CH1-XL RoboColumn

Catalog number: 5943462200
Catalog Number
5943462200
Unit Size
200 µL
Price (USD)
376.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
600 µL
Price (USD)
444.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
5943462200200 µL
376.00
Your Price:
***
5943462600600 µL
444.00
Your Price:
***
Product Overview
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CaptureSelect RoboColumn™ small chromatography columns are used for fully automated and parallel chromatographic separations on robotic liquid handling workstations.

CaptureSelect CH1-XL Affinity Matrix recognizes the CH1 domain of human IgG antibodies, thereby enabling purification of all Fab fragments, independent of the light-chain type (kappa or lambda) and without binding of over-expressed light chain and light-chain dimers. In addition, it can be used for the purification of human IgGs from all four subclasses (1, 2, 3, and 4).

Features of this affinity matrix include:
• Platform approach for purification of high quality and correctly assembled human Fab fragments
• No co-purification of over-expressed free light chains due to selectivity for the CH1 domain
• Elution possible at pH 4.0–4.5 with 50 mM acetate buffer
• Caustic cleaning with up to 50 mM NaOH
• Excellent scalability
• No cross-binding to bovine IgG, therefore presence of fetal bovine serum is not a problem
• Non-animal-derived

Free of animal components
CaptureSelect affinity products are created by a proprietary technology based on camelid-derived single domain antibody fragments. The CH1-XL ligand is a 13-kDa single domain fragment comprising the three CDRs that form the antigen-binding domain, efficiently produced in Saccharomyces cerevisiae in a production process free of animal components.

Characteristics

Column size: 200 µL and 600 µL, 1 strip, 8 columns (8-row)
Column dimension: 200 uL: 5 mm D x 10 mm L
Column dimension: 600 uL: 5 mm D x 30 mm L
Matrix: agarose based, epoxide-activated
Average particle size: 65 ± 10 µm
Ligand: CaptureSelect CH1-XL affinity ligand
Ligand coupling: epoxide coupling
Binding capacity: >15 mg/mL human Fab fragment depending on flow rate, column height, contact time. For humanized Fabs, lower capacities may be observed.
Elution conditions: 100 mM glycine buffer, pH 3.0; 50 mM acetate buffer, pH 4.0–4.5
Formulation buffer: 20% (v/v) ethanol
Operating pressure: 2 bar (maximum)
Flow rate: 16–150 cm/h

RoboColumn is a trademark of Repligen.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Application)
Chromatography
Stationary Phase
CH1-XL affinity ligand on agarose bead
Form
8-well Strips
Product Line
CaptureSelect™
Type
RoboColumn
Binding Capacity
>15 mg/mL
Capacity (Metric)
200 μL
Flow Rate
19 to 150 cm/hr.
Matrix
Agarose-based, Epoxide-activated
Diameter (Metric)
5 mm
Max. Operating Pressure
2 bar (Max)
Length (Metric)
10 mm
Shipping Condition
Room temperature, or on wet ice
Particle Size
65 ± 10 micron

Contents & Storage

Store in refrigerator (2°C to 8°C)

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