Thermo Scientific™

CaptureSelect™ CH1-XL RoboColumn

Catalog number: 5943462200
Thermo Scientific™

CaptureSelect™ CH1-XL RoboColumn

Catalog number: 5943462200
Catalog Number
5943462200
Unit Size
200 µL
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
600 µL
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
5943462200200 µL
-
5943462600600 µL
-
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CaptureSelect RoboColumn™ small chromatography columns are used for fully automated and parallel chromatographic separations on robotic liquid handling workstations.

CaptureSelect CH1-XL Affinity Matrix recognizes the CH1 domain of human IgG antibodies, thereby enabling purification of all Fab fragments, independent of the light-chain type (kappa or lambda) and without binding of over-expressed light chain and light-chain dimers. In addition, it can be used for the purification of human IgGs from all four subclasses (1, 2, 3, and 4).

Features of this affinity matrix include:

  • Platform approach for purification of high quality and correctly assembled human Fab fragments
  • No co-purification of over-expressed free light chains due to selectivity for the CH1 domain
  • Elution possible at pH 4.0–4.5 with 50 mM acetate buffer
  • Caustic cleaning with up to 50 mM NaOH
  • Excellent scalability
  • No cross-binding to bovine IgG, therefore presence of fetal bovine serum is not a problem
  • Non-animal-derived

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Characteristics

  • Column size: 200 μL and 600 μL, 1 strip, 8 columns (8-row)
  • Column dimension: 200 uL: 5 mm D x 10 mm L
  • Column dimension: 600 uL: 5 mm D x 30 mm L
  • Matrix: agarose based, epoxide-activated
  • Average particle size: 65 ± 10 μm
  • Ligand: CaptureSelect CH1-XL affinity ligand
  • Ligand coupling: epoxide coupling
  • Binding capacity: >15 mg/mL human Fab fragment depending on flow rate, column height, contact time. For humanized Fabs, lower capacities may be observed.
  • Elution conditions: 100 mM glycine buffer, pH 3.0; 50 mM acetate buffer, pH 4.0–4.5
  • Formulation buffer: 20% (v/v) ethanol
  • Operating pressure: 2 bar (maximum)
  • Flow rate: 16–150 cm/h

RoboColumn is a trademark of Repligen.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Binding Capacity
>15 mg/mL human Fab fragment
Capacity (Metric)
200 μL
Flow Rate
16 to 150 cm/hr.
Matrix
Agarose-based, epoxide-activated
Diameter (Metric)
5 mm
Max. Operating Pressure
2 bar
Length (Metric)
10 mm
Shipping Condition
Room temperature, or on wet ice
Particle Size
65 ± 10 μm
Product Line
CaptureSelect™
For Use With (Application)
Chromatography
Type
Column
Ligand Type
CH1-XL affinity ligand
Stationary Phase
CH1-XL affinity ligand on agarose bead
Format
8-well Strips
Column Type
RoboColumn

Contents & Storage

Store in refrigerator (2°C to 8°C)

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