CaptureSelect RoboColumn™ small chromatography columns are used for fully automated and parallel chromatographic separations on robotic liquid handling workstations.
CaptureSelect CH1-XL Affinity Matrix recognizes the CH1 domain of human IgG antibodies, thereby enabling purification of all Fab fragments, independent of the light-chain type (kappa or lambda) and without binding of over-expressed light chain and light-chain dimers. In addition, it can be used for the purification of human IgGs from all four subclasses (1, 2, 3, and 4).
Features of this affinity matrix include: • Platform approach for purification of high quality and correctly assembled human Fab fragments • No co-purification of over-expressed free light chains due to selectivity for the CH1 domain • Elution possible at pH 4.0–4.5 with 50 mM acetate buffer • Caustic cleaning with up to 50 mM NaOH • Excellent scalability • No cross-binding to bovine IgG, therefore presence of fetal bovine serum is not a problem • Non-animal-derived
Free of animal components CaptureSelect affinity products are created by a proprietary technology based on camelid-derived single domain antibody fragments. The CH1-XL ligand is a 13-kDa single domain fragment comprising the three CDRs that form the antigen-binding domain, efficiently produced in Saccharomyces cerevisiae in a production process free of animal components.
Column size: 200 µL and 600 µL, 1 strip, 8 columns (8-row) Column dimension: 200 uL: 5 mm D x 10 mm L Column dimension: 600 uL: 5 mm D x 30 mm L Matrix: agarose based, epoxide-activated Average particle size: 65 ± 10 µm Ligand: CaptureSelect CH1-XL affinity ligand Ligand coupling: epoxide coupling Binding capacity: >15 mg/mL human Fab fragment depending on flow rate, column height, contact time. For humanized Fabs, lower capacities may be observed. Elution conditions: 100 mM glycine buffer, pH 3.0; 50 mM acetate buffer, pH 4.0–4.5 Formulation buffer: 20% (v/v) ethanol Operating pressure: 2 bar (maximum) Flow rate: 16–150 cm/h
RoboColumn is a trademark of Repligen.
For Research Use Only. Not for use in diagnostic procedures.