Thermo Scientific™

SMART Digest™ Pepsin Kits

Catalog number: 60109-110-B
Thermo Scientific™

SMART Digest™ Pepsin Kits

Catalog number: 60109-110-B

Use Thermo Scientific™ SMART Digest™ Pepsin Kits in your peptide mapping and peptide quantitation workflows to obtain high quality analytical results. A significant advance in sample preparation for biopharmaceutical protein research, the kits provide fast and simple protein digestion with high reproducibility, high sensitivity, and high levels of data quality in a format that's compatible with automation. The SMART Digest Pepsin Kits are a significant improvement over current in-solution protein digestion technologies, which are not reproducible, have poor sensitivity, and employ protracted methodologies not amenable to automation.

 
Catalog Number
60109-110-B
Unit Size
Each
Description
SMART Digest Pepsin Kit, Bulk Resin (No Collection Plate)
Price (USD)
684.00
Your Price:
Availability
***
Full specifications
Quantity
TypeProtein Digestion
DescriptionSMART Digest Pepsin Kit, Bulk Resin (No Collection Plate)
IncludesBulk Non-magnetic SMART Digest Resin, SMART Digest Buffer
Product LineSMART Digest
For Use With (Application)Peptide Quantitation, Peptide Mapping
FormatSMART Digest Resin, Bulk Non-magnetic
EnzymePepsin
CaptureNone
CleavageHydrophobic residues in P1 and P1' positions at the carboxyl side of phenylalanine and leucine and at the carboxyl side of glutamic acid
Unit SizeEach
Catalog Number
Unit Size
Each
Description
SMART Digest Pepsin Kit with Collection Plate
Price (USD)
712.00
Your Price:
Availability
***
Full specifications
Quantity
TypeProtein Digestion
DescriptionSMART Digest Pepsin Kit with Collection Plate
IncludesSMART Digest Resin Pre-filled into 96 PCR Tubes, Collection Plate
Product LineSMART Digest
For Use With (Application)Peptide Quantitation, Peptide Mapping
FormatSMART Digest Resin Pre-filled into PCR Tubes
EnzymePepsin
CaptureNone
CleavageHydrophobic residues in P1 and P1' positions at the carboxyl side of phenylalanine and leucine and at the carboxyl side of glutamic acid
Unit SizeEach
Catalog Number
Unit Size
Each
Description
SMART Digest Pepsin Kit with Filter and Collection Plate
Price (USD)
828.00
Your Price:
Availability
***
Full specifications
Quantity
TypeProtein Digestion
DescriptionSMART Digest Pepsin Kit with Filter and Collection Plate
IncludesSMART Digest Resin Pre-filled into 96 PCR Tubes, Collection Plate, Filter Plate
Product LineSMART Digest
For Use With (Application)Peptide Quantitation, Peptide Mapping
FormatSMART Digest Resin Pre-filled into PCR Tubes
EnzymePepsin
CaptureNone
CleavageHydrophobic residues in P1 and P1' positions at the carboxyl side of phenylalanine and leucine and at the carboxyl side of glutamic acid
Unit SizeEach
Catalog Number
Unit Size
Each
Description
SMART Digest Pepsin Kit with SOLAµ Collection Plate
Price (USD)
842.00
Your Price:
Availability
***
Full specifications
Quantity
TypeProtein Digestion
DescriptionSMART Digest Pepsin Kit with SOLAµ Collection Plate
IncludesSMART Digest Resin Pre-filled into 96 PCR Tubes, Collection Plate, SOLAµ SPE Plate
Product LineSMART Digest
For Use With (Application)Peptide Quantitation, Peptide Mapping
FormatSMART Digest Resin Pre-filled into PCR Tubes
EnzymePepsin
CaptureNone
CleavageHydrophobic residues in P1 and P1' positions at the carboxyl side of phenylalanine and leucine and at the carboxyl side of glutamic acid
Unit SizeEach
Catalog Number
Unit Size
Each
Description
SMART Digest Pepsin Kit with Magnetic Bead, Bulk Option (No Collection Plate)
Price (USD)
692.00
Your Price:
Availability
***
Full specifications
Quantity
TypeProtein Digestion
DescriptionSMART Digest Pepsin Kit with Magnetic Bead, Bulk Option (No Collection Plate)
IncludesBulk Magnetic SMART Digest Resin, SMART Digest Buffer
Product LineSMART Digest
For Use With (Application)Peptide Quantitation, Peptide Mapping
FormatSMART Digest Resin, Bulk Magnetic
EnzymePepsin
CaptureNone
CleavageHydrophobic residues in P1 and P1' positions at the carboxyl side of phenylalanine and leucine and at the carboxyl side of glutamic acid
Unit SizeEach
Showing 5 of 5
Catalog NumberSpecificationsUnit SizeDescriptionPrice (USD)AvailabilityQuantity
60109-110-BFull specifications
EachSMART Digest Pepsin Kit, Bulk Resin (No Collection Plate)
684.00
Your Price:
***
TypeProtein Digestion
DescriptionSMART Digest Pepsin Kit, Bulk Resin (No Collection Plate)
IncludesBulk Non-magnetic SMART Digest Resin, SMART Digest Buffer
Product LineSMART Digest
For Use With (Application)Peptide Quantitation, Peptide Mapping
FormatSMART Digest Resin, Bulk Non-magnetic
EnzymePepsin
CaptureNone
CleavageHydrophobic residues in P1 and P1' positions at the carboxyl side of phenylalanine and leucine and at the carboxyl side of glutamic acid
Unit SizeEach
60109-110Full specifications
EachSMART Digest Pepsin Kit with Collection Plate
712.00
Your Price:
***
TypeProtein Digestion
DescriptionSMART Digest Pepsin Kit with Collection Plate
IncludesSMART Digest Resin Pre-filled into 96 PCR Tubes, Collection Plate
Product LineSMART Digest
For Use With (Application)Peptide Quantitation, Peptide Mapping
FormatSMART Digest Resin Pre-filled into PCR Tubes
EnzymePepsin
CaptureNone
CleavageHydrophobic residues in P1 and P1' positions at the carboxyl side of phenylalanine and leucine and at the carboxyl side of glutamic acid
Unit SizeEach
60109-111Full specifications
EachSMART Digest Pepsin Kit with Filter and Collection Plate
828.00
Your Price:
***
TypeProtein Digestion
DescriptionSMART Digest Pepsin Kit with Filter and Collection Plate
IncludesSMART Digest Resin Pre-filled into 96 PCR Tubes, Collection Plate, Filter Plate
Product LineSMART Digest
For Use With (Application)Peptide Quantitation, Peptide Mapping
FormatSMART Digest Resin Pre-filled into PCR Tubes
EnzymePepsin
CaptureNone
CleavageHydrophobic residues in P1 and P1' positions at the carboxyl side of phenylalanine and leucine and at the carboxyl side of glutamic acid
Unit SizeEach
60109-112Full specifications
EachSMART Digest Pepsin Kit with SOLAµ Collection Plate
842.00
Your Price:
***
TypeProtein Digestion
DescriptionSMART Digest Pepsin Kit with SOLAµ Collection Plate
IncludesSMART Digest Resin Pre-filled into 96 PCR Tubes, Collection Plate, SOLAµ SPE Plate
Product LineSMART Digest
For Use With (Application)Peptide Quantitation, Peptide Mapping
FormatSMART Digest Resin Pre-filled into PCR Tubes
EnzymePepsin
CaptureNone
CleavageHydrophobic residues in P1 and P1' positions at the carboxyl side of phenylalanine and leucine and at the carboxyl side of glutamic acid
Unit SizeEach
60109-110-MBFull specifications
EachSMART Digest Pepsin Kit with Magnetic Bead, Bulk Option (No Collection Plate)
692.00
Your Price:
***
TypeProtein Digestion
DescriptionSMART Digest Pepsin Kit with Magnetic Bead, Bulk Option (No Collection Plate)
IncludesBulk Magnetic SMART Digest Resin, SMART Digest Buffer
Product LineSMART Digest
For Use With (Application)Peptide Quantitation, Peptide Mapping
FormatSMART Digest Resin, Bulk Magnetic
EnzymePepsin
CaptureNone
CleavageHydrophobic residues in P1 and P1' positions at the carboxyl side of phenylalanine and leucine and at the carboxyl side of glutamic acid
Unit SizeEach
Showing 5 of 5

How SMART Digest Pepsin Kits work
Pepsin cleaves proteins preferentially at carboxylic groups of aromatic amino acids such as phenylalanine, tryptophan and tyrosine. It will not cleave at bonds containing valine, alanine or glycine. Pepsin is often used to generate antibody fragments. Pepsin is used to generate F(ab')2 fragments by removing only that portion of the Fc domain beyond the hinge region. The resulting F(ab')2 fragments are composed of two antibody-binding Fab' fragments connected by disulfides in the hinge region.

Figures

Frequently asked questions (FAQs)

Citations & References