SLE offers a number of advantages over existing sample preparation techniques such as Liquid/Liquid Extraction (LLE) and Protein Precipitation techniques for dealing with biological matrices. In comparison to LLE, SLE offers greater reproducibility and recoveries, lower solvent requirements and prevents emulsification which is often associated with the LLE technique. It also lends itself to full automation which is not possible via LLE. SLE provides improved cleanliness of sample extract and associated increase in sensitivity compared to protein precipitation techniques which are unable to remove phospholipids.
- Simple, easy-to-use protocol
- Removal of phospholipids
- Greater reproducability and recoveries compared to LLE
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