Thermo Scientific™

SMART Digest™ ImmunoAffinity (IA) Streptavidin Kits

 Related applications:
Industrial Chromatography

Obtain fast, simple, highly reproducible, and sensitive immunocapture and protein digestion in a single well using Thermo Scientific™ SMART Digest™ ImmunoAffinity (IA) Streptavidin Kits. These kits were designed to remove the challenges often associated with the process of biomarker quantitation by providing a simple and easy capture-digestion workflow that takes only hours instead of overnight. The kit purifies various target biomolecules via the formation of strong streptavidin-biotin bond.

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Description

Solving the Challenges of Low-level Quantitation in Complex Matrices

The need to improve the ability to identify low-level biomarkers is forcing the analytical community to deal with extremely high levels of analyte and sample complexity, which makes quantitation challenging.

As these proteins are often present at low levels in complex biological matrices, an immunoaffinity capture step is typically employed to purify the sample and increase sensitivity. This step is often followed by protein digestion. Current processes are often time consuming, multistep, and prone to irreproducibility.

Immunocapture and Protein Digestion in a Single Well

These IA kits deliver a process that is:

  • Fast
  • Simple
  • Highly reproducible
  • Sensitive
Unique Design Reduces Workflow to Hours

In SMART Digest IA Kits, the immunocapture reagents and the temperature-activated, thermally stable trypsin are co-immobilized onto a resin. Following enrichment, the enzyme is activated at elevated temperatures for accelerated digestion under protein denaturing conditions, resulting in a workflow which takes hours rather than overnight.

Putting Peptides on the Map

Putting Peptides on the Map

How do advances in digestion, separation, mass detection & data interpretation remove roadblocks in protein characterization?

Download eBook ›



Faster and More Sensitive Protein Characterization and Quantitation

Faster and More Sensitive Protein Characterization and Quantitation

How can characterization and quantitation of bio-therapeutics can be made easier, faster and more reproducible?

Download eBook ›

Documents

Manuals & protocols