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Thermo Scientific™
SMART Digest™ Dual Enzyme Columns for HDX-MS Analysis
Thermo Scientific™ SMART Digest™ Dual Enzyme Columns for HDX-MS analysis provide fast, reliable digestion of proteins for HDX-MS analysis, helping to provide the sequence characterization of biotherapeutics.
| Catalog Number | Particle Size |
|---|---|
| 60160-110 | ˜20 μm |
Catalog number 60160-110
Price (KRW)
-
Particle Size:
˜20 μm
Thermo Scientific SMART Digest Dual Enzyme columns containing pepsin and protease XIII provide fast, reliable digestion of proteins for for HDX-MS analysis helping to provide the sequence characterization of biotherapeutics.
SMART Digest Dual Enzyme columns for HDX-MS analysis help to provide the characterization of biotherapeutics.
The combination of enzymes are complementary, creating many short, overlapping N-terminal peptides for a high degree of sequence coverage.
Pepsin cleaves proteins preferentially at carboxylic groups of aromatic amino acids such as phenylalanine, tryptophan and tyrosine. It will not cleave at bonds containing valine, alanine or glycine. The optimum pH range for pepsin activity is 1.0–3.0.
Proteinase XIII cleaves proteins preferentially at carboxylic groups of basic amino acids (arginine, lysine, histidine). Like pepsin, Proteinase XIII is active at low pH.
Specifications
Column FormatImmobilized enzyme
Column TypeImmobilized enzyme in PEEK cartridge
DescriptionSMART Digest Pepsin/Protease XIII Dual Enzyme Column
Diameter2.1 mm
Flow Rate10 to 500 μL/min
For Use With (Application)HDX-MS analysis
Length30 mm
Max. Pressure2500 psi (170 bar)
Mobile Phase CompatibilityPhosphate buffer pH 4
Operating Temperature Range4 °C to 50 °C
Packing MaterialHydrophilic/coated DVB
Particle Size˜20 μm
Pore Size° 1000 angstroms
Stability1 year
Stationary PhaseImmobilized enzyme
Storage TemperatureRT for up to 2 weeks; 4 °C for extended period (refrigerate in MES pH 6)
TechniqueBioLC
Weight8 g
CleavagePepsin: Cleaves proteins preferentially at carboxylic groups of aromatic amino acids such as phenylalanine, tryptophan and tyrosine. The optimum pH range for pepsin activity is 1.0–3.0.
Protease XIII: Cleaves proteins preferentially at carboxylic groups of basic amino acids (arginine, lysine, histidine). Like pepsin, Proteinase XIII is active at low pH.
Protease XIII: Cleaves proteins preferentially at carboxylic groups of basic amino acids (arginine, lysine, histidine). Like pepsin, Proteinase XIII is active at low pH.
EnzymePepsin & Protease XIII
TypeSMART Digest Pepsin/Protease XIII Dual Enzyme column
pH2 to 6
Unit SizeEach