Platinum® Tfi Exo(-) DNA Polymerase

Catalog number: 60684050

Invitrogen™  Related applications: PCR

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Description

Platinum® Tfi Exo(-) DNA Polymerase is recombinant Tfi Exo(-) DNA Polymerase complexed with a proprietary antibody mix that inhibits polymerase activity at ambient temperature, allowing room-temperature reaction setup. Activity is restored after the initial denaturation step in PCR cycling at 94°C, providing an automatic "hot start" for increased specificity, sensitivity, and yield. Tfi Exo(-) DNA Polymerase is purified from E. coli. expressing cloned mutants of the Thermus filiformis DNA polymerase gene. This enzyme has 5'→3' polymerase activity, but lacks both 5'→3' and 3'→5' exonuclease activity for improved yield and robustness. Tfi Exo(-) DNA polymerase is heat-stable and will synthesize DNA at elevated temperatures from single-stranded templates in the presence of a primer.

Applications
Platinum® Tfi Exo(-) DNA Polymerase can be used in protocols that currently use Platinum® Taq DNA Polymerase without modification. PCR performance is comparable to that of Platinum® Taq in yield, specificity, fidelity, and robustness. Like Platinum® Taq, Platinum® Tfi Exo(-) DNA Polymerase has a nontemplate-dependent terminal transferase activity that adds a single deoxyadenosine (A) to the 3' ends of PCR products.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Polymerase: Tfi DNA Polymerase
Hot Start: Built-In Hot Start
Fidelity (vs. Taq): 1 X
GC-Rich PCR Performance: Low
Reaction Format: Separate Components
Product Overhang: 3'-A
Reaction Speed: Standard
Final Product Size(s): 1 kb or less
Product Size: 500 units
Shipping Condition: Dry Ice

Contents & storage

Platinum® Tfi DNA Polymerase (500 units total, at 5U/µL) is supplied with 5X Platinum® Tfi Reaction Buffer (4 × 1.3 mL) and 50 mM Magnesium Chloride (1 mL). Store at -20°C (non-frost-free).

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