In-Cell ELISA Near Infrared Detection Kit

Catalog number: 62201

Thermo Scientific™  Related applications: Protein Assays and Analysis

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The Thermo Scientific In-Cell ELISA Near Infrared Detection Kit is a simple and convenient method for quantification of intracellular protein levels in whole cells.

Features of the In-Cell ELISA Near Infrared Detection Kit:

• Near Infrared—compatible with the LI-COR Odyssey Infrared Imaging System
• Simple, fast assay format
• Ability to perform multiplex experiments
• No special sample preparation required
• Higher throughput compared with western blotting
• Cost efficient (one well vs. one lane) compared to western blotting

While traditional western blot analysis is time consuming and only semi-quantitative, the in-cell ELISA is an accurate method to determine relative protein levels and degree of post-translational modification (PTM) among various cell types. The In-cell ELISA may be performed in 96- or 384-well microplates, is scalable and conserves cell culture and treatment reagents. Because assay data are measured using a standard ELISA plate reader or infrared imaging system, the results are readily available for analysis. Also, the assay is amenable to automation, which is ideal for siRNA studies and drug screens.

• High throughput determination of relative protein expression levels
• Monitor dose-dependent post-translational protein modification
• Multiplex analysis of targets in multiple cell lines

The In-Cell ELISA Near IR Kit enables simultaneous detection of two different targets within the same well (e.g., PTM- and unmodified-forms of a protein, or two entirely different proteins). Simultaneously detecting targets within the same microplate well eliminates variability caused by differences in cell plating. The expression levels of the protein(s) are monitored using primary antibodies specific to the targets and corresponding species-specific Near Infrared DyLight Fluor-Conjugated Secondary Antibodies. Relative protein activation or inactivation is determined as a ratio of modified protein levels to the corresponding unmodified protein measured using a modification-specific antibody and an antibody to unmodified protein, respectively.

Related Products
In-Cell ELISA Colorimetric Detection Kit
For Research Use Only. Not for use in diagnostic procedures.


Species: Multiple
Product Size: 4 x 96 test kit
Protein Family: Other Proteins
Detection Method: Colorimetric
Research Category: Immunology

Contents & storage

Sufficient For: 4 x 96-well microplates of overnight-cultured cells
• Blocking Buffer, 100 mL
• 20X Tris Buffered Saline, 50 mL
• Surfact-Amps 20 (10% Tween-20 Detergent), 10 mL
• Surfact-Amps X-100 (10% Triton X-100 Detergent), 10 mL
• DyLight 680 Goat Anti-Mouse IgG (H+L), 55 µL
• DyLight 800 Goat Anti-Rabbit IgG (H+L), 55 µL
• Thin Plate Seal Assembly, 8 each

Store antibodies as indicated on vials. Store remaining components at 4°C.


Manuals & protocols