STAT6 Colorimetric In-Cell ELISA Kit, Multispecies

Catalog number: 62208

Thermo Scientific™  Related applications: Protein Assays and Analysis

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Description

Thermo Scientific Pierce STAT6 Colorimetric In-Cell ELISA Kit is a colorimetric microplate assay optimized to measure total and phosphorylated signal transducers and activators of transciption 6 (phospho-STAT6) in whole cells.

Features of STAT6 Colorimetric In-Cell ELISA Kit:

• Measures total and phosphorylated signal transducers and activators of transciption 6 (STAT6) in whole cells using a 96-well microplates
• Reliable colorimetric detection kit is available for use with standard microplate readers
• Whole-cell detection requires no cell lysis, protein extraction or special sample preparation
• Provides better data and higher throughput than quantitative Western blotting
• Less costly (one well vs. one lane) than Western blotting

STAT6 In-Cell ELISA Kits for whole cells include a mouse monoclonal anti-STAT6 antibody, rabbit monoclonal anti-phospho-(Tyr641) STAT6 antibody, detection reagents and all buffers required for either colorimetric or near infrared detection. STAT6 In-Cell ELISA Kits enable simultaneous detection of both total and phospho-(Tyr641) STAT6.

Overview of STAT6 and phosphorylation of STAT6:
STAT6 belongs to the STAT family of transcription factors which regulate many aspects of cell growth, survival and differentiation. Inactive STAT proteins are located in the cytosol until phosphorylated by receptor associated kinases. Upon phosphorylation, STAT proteins form homodimers or heterodimers with other STAT proteins and translocate into the cell nucleus. STAT6 is known interact with the transcriptional regulators EP300, CREB, NFκB and is integral in the anti-apoptotic effects of IL-4 through regulation of BCL2L1/BCL-X(L) expression.

Related Products
Pierce™ STAT6 Near Infrared In-Cell ELISA Kit
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Species: Multiple
Product Size: 96 assays
Protein Family: Other Proteins
Detection Method: Colorimetric
Research Category: Signal Transduction

Contents & storage

Sufficient For: 96 microplate wells of overnight-cultured cells
• Blocking Buffer, 50 mL
• 20X Tris Buffered Saline, 50 mL
• Surfact-Amps 20 (10% Detergent), 10 mL
• Surfact-Amps X-100 (10% Detergent), 10 mL
• HRP Conjugate, 0.14 mL
• TMB Substrate, 58 mL
• TMB Stop Solution, 55 mL
• Janus Green Whole-Cell Stain, 50 mL
• Elution Buffer, 2 x 30 mL
• Thin Plate Seal Assembly, 8 each
• Anti-Phospho STAT6 (Y641) Antibody
• Anti-STAT6 Antibody

Store antibodies as indicated on vial. Store remaining components at 4°C.

Documents

Manuals & protocols