STAT3 Colorimetric In-Cell ELISA Kit, Multispecies

Catalog number: 62209

Thermo Scientific™  Related applications: Protein Assays and Analysis

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Description

Thermo Scientific Pierce STAT3 Colorimetric In-Cell ELISA Kit is a colorimetric microplate assays optimized to measure total and phosphorylated signal transducers and activators of transcription 3 (phospho-STAT3) in whole cells.

Features of STAT3 Colorimetric In-Cell ELISA Kit:

• Measures total and phosphorylated signal transducers and activators of transcription 3 (STAT3) in whole cells using a 96-well microplates
• Reliable colorimetric detection kit for use with standard microplate readers
• Whole-cell detection requires no cell lysis, protein extraction or special sample preparation
• Provides better data and higher throughput than quantitative Western blotting
• Less costly (one well vs. one lane) than Western blotting

STAT3 In-Cell ELISA kit for whole cells include a mouse monoclonal anti-STAT3 antibody, rabbit monoclonal anti-phospho-(Tyr705) STAT3 antibody, detection reagents and all buffers required for either colorimetric or near infrared detection. The STAT3 In-Cell ELISA Kits enable simultaneous detection of both total and phospho-(Tyr705) STAT3.

Overview of STAT3 and phosphorylation of STAT3:
STAT3 belongs to the STAT family of transcription factors which regulate many aspects of cell growth, survival and differentiation. Inactive STAT proteins are located in the cytosol until phosphorylated by receptor associated kinases. Upon phosphorylation, STAT proteins form homodimers or heterodimers with other STAT proteins and translocate into the cell nucleus. STAT3 is activated in response to cytokines and growth factors such as interons, epidermal growth factors, interleukins and bone morphogenetic protein 2. STAT3 is known to be regulated by Rac1, PIAS3, JAK2 and c-MET. Activation of STAT3 is required for embyronic stem cell survial and development of mouse embryos past embyronic day 7. STAT3 is also implicated in human cancers.

Related Products
Pierce™ STAT3 Near Infrared In-Cell ELISA Kit
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Species: Multiple
Product Size: 96 test kit
Protein Family: Other Proteins
Detection Method: Colorimetric
Research Category: Signal Transduction

Contents & storage

Sufficient For: 96 microplate wells of overnight-cultured cells
• Blocking Buffer, 50 mL
• 20X Tris Buffered Saline, 50 mL
• Surfact-Amps 20 (10% Detergent), 10 mL
• Surfact-Amps X-100 (10% Detergent), 10 mL
• HRP Conjugate, 0.14 mL
• TMB Substrate, 58 mL
• TMB Stop Solution, 55 mL
• Janus Green Whole-Cell Stain, 50 mL
• Elution Buffer, 2 x 30 mL
• Thin Plate Seal Assembly, 8 each
• Anti-Phospho STAT3 (Y705) Antibody
• Anti-STAT3 Antibody

Store antibodies as indicated on vial. Store remaining components at 4°C.

Documents

Manuals & protocols