Thermo Scientific™

5-IAF (5-iodoacetamido-fluorescein)

Catalog number:  62246

Related applications:

Protein Labeling & Crosslinking

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Thermo Scientific 5-Iodoacetamido-Fluorescein (5-IAF) is a high-performance derivative of fluorescein dye, activated for easy and reliable labeling of antibodies, proteins and other molecules for use as fluorescent probes. 5-Iodoacetamido-Fluorescein is a sulfhydryl-reactive derivative of fluorescein that labels proteins and other molecules having free thiols (cysteine side chains)

Properties of 5-Iodoacetamido-Fluorescein:

• Alternative names: 5-IAF, 5-iodoacetamidofluorescein
• Chemical name: Acetamide, N-(3',6'-dihydroxy-3-oxospiro (isobenzofuran-1(3H), 9'-(9H)xanthen)-5-yl)-2-iodo
• Molecular weight: 515.26±3
• Excitation source: 488 nm spectral line, argon-ion laser
• Excitation wavelength: 494 nm
• Emission wavelength: 518 nm
• Extinction coefficient: > 80,000 M-1cm-1
• CAS #: 63368-54-7
• Solubility: Soluble in DMF; aqueous buffers at pH > 6
• Reactive groups: Iodoacetamide, reacts with sulfhydryls at pH 7.0 to 7.5

• Label antibodies for use as immunofluorescent probes
• Label oligonucleotides for hybridization probes
• Detect proteins in gels and on Western blots

Related Products
For Research Use Only. Not for use in diagnostic procedures.


Chemical Reactivity: Thiol
Excitation/Emission: 494⁄518
Label Type: Classic Dyes
Label or Dye: Fluorescein (FITC), FITC (Fluorescein)
Product Line: DyLight™
Reactive Moiety: Haloacetyl
Solubility: DMF (Dimethylformamide)

Contents & storage

Store desiccated at -20°C, protect from moisture