Thermo Scientific™

DAPI Solution (1 mg/mL)

Catalog number: 62248
Thermo Scientific™

DAPI Solution (1 mg/mL)

Catalog number: 62248
Catalog Number
Unit Size
1 mL
Price (USD)
Price: 166.00
Online Offer: 154.65
(ends 31-Dec-2023)
Your Price:
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
622481 mL
Price: 166.00
Online Offer: 154.65
(ends 31-Dec-2023)
Your Price:
Product Overview
Citations & References
Additional Information
Thermo Scientific Pierce DAPI Nuclear Counterstains are high-purity forms of diamidino-2-phenylindole dye for fixed-cell, fluorescent staining of DNA content and nuclei for cellular imaging techniques.

Also available as a room-temperature-stable, ready-to-use solution: NucBlue Fixed Cell Ready Probes Reagent.
See other Ready Probes ready-to-use imaging reagents and accessories ›

DAPI (diamidino-2-phenylindole) is a blue fluorescent probe that fluoresces brightly upon selectively binding to the minor groove of double stranded DNA, where its fluorescence is approximately 20-fold greater than in the nonbound state. Its selectivity for DNA and high cell permeability allows efficient staining of nuclei with little background from the cytoplasm. DAPI is a classic nuclear counterstain for immunofluorescence microscopy, as well as an important component of high-content screening methods requiring cell-based quantitation of DNA content.

Features of DAPI Nuclear Counterstain:

DAPI dye—diamidino-2-phenylindole is a blue fluorescent stain specific for DNA
Convenient—choose powder or easy-to-use DAPI dye solution (1 mg/mL)
Cellular imaging—cell-permeable dye is effective for fixed-cell staining and quantitation of DNA content
Counterstain—ideal for use alongside detection of specific targets with fluorescent antibodies for fluorescence microscopy or high-content screening (HCS)

Properties of DAPI Fluorescent Dye:

Alternative names: DAPI Stain, DAPI Dye, DNA Content Counterstain
Chemical name(s): 4,6'-diamidino-2-phenylindole, dihydrochloride; 4',6-diamidine-2-phenyl indole; 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride
Molecular formula: C16H15N5·2HCl
Molecular weight: 350.25
Excitation wavelength: 341±3 nm (near 360 nm when bound to dsDNA)
Emission wavelength: 452±3 nm (456 to 460 nm when bound to dsDNA)
Extinction coefficient: >30,600/M cm at 347 nm in methanol
CAS #: 28718-90-3
Purity: > 95% (most lots >98%) by HPLC at 240 nm
Solubility: >1 mg/mL in water; compound is soluble in DMF, water and various non-phosphate aqueous buffers
Reactive groups: None; binds to minor groove of double-stranded DNA

As a counterstain in fluorescence imaging methods, DAPI is compatible with antibodies and other probes labeled with fluorescein and rhodamine dyes, as well as with Thermo Scientific DyLight Fluors. DAPI has greater photostability than Hoechst dyes, although Hoechst 33342 can be use for live cell imaging while use of DAPI is confined to fixed cells. DAPI is offered in powdered solid and aqueous solution forms.

Applications for DAPI Stain:

• Assaying DNA in solution (ref.4)
• Diagnosing mycoplasmal infection of cell cultures (ref.5)
• Measuring nuclear content and sorting chromosomes in flow cytometry (ref.6)
• Assessing apoptosis (ref.7)
• Detecting nuclei and organellar DNA in immunofluorescent and in situ hybridization procedures (ref.2,8)
• Replacing ethidium bromide for staining DNA in agarose gels (ref.5,9)
• Counterstaining nuclei in histochemical methods when red-fluorescent antibodies have been used to detect specific targets (ref.8)
• Reports also indicate that DAPI will bind to polyphosphates and other polyanions (ref.10), dextran sulfate (ref.11) and SDS (ref.12).

Related Products
DAPI (4',6-diamidino-2-phenylindole, dihydrochloride)
For Research Use Only. Not for use in diagnostic procedures.


Excitation Wavelength Range
360 nm
Dye Type
For Use With (Application)
Cell imaging
For Use With (Equipment)
Fluorescence Microscope, High Content Instrument
1 mL
Detection Method
DAPI (4',6-diamidino-2-phenylindole, dihydrochloride)
460 nm
Product Line
Shipping Condition
Room Temperature
Product Type
SubCellular Localization

Contents & Storage

Store DAPI solution (1 mg/mL) at 2–8°C and protect from light.


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