Thermo Scientific™

DRAQ5™ Fluorescent Probe Solution (5 mM)

Catalog number: 62254
Thermo Scientific™

DRAQ5™ Fluorescent Probe Solution (5 mM)

Catalog number: 62254
Catalog Number
62254
Unit Size
50 µL
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
200 µL
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
1 mL
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
6225450 µL
-
62251200 µL
-
622521 mL
-
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The Thermo Scientific DRAQ5 Fluorescent Probe is a far-red DNA stain for live or fixed cells and easy multiplexing with other fluorophores, such as cells expressing green fluorescent protein (GFP) fusion proteins.

DRAQ5 Fluorescent Probe is a far-red DNA stain for fluorescent cellular imaging applications with live or fixed cells. Because of its far-red excitation and emission, the DRAQ5 Stain can be multiplexed with many other fluorophores. It is commonly chosen to counterstain nuclei and measure DNA content in cell proliferation studies involving cells expressing green fluorescent protein (GFP) fusion proteins. DRAQ5 Fluorescent Probe is compatible with many existing protocols across a wide range of instrumentation platforms.

Features of the DRAQ5 Fluorescent Probe:

DRAQ5 dye—a red-to-infrared fluorescent stain specific for DNA (nuclei and DNA quantitation)
Convenient—provided as easy-to-use DRAQ5 dye solutions (5 mM)
Cellular imaging—lipophilic and membrane permeable dye is effective for live-cell and fixed-cell staining and quantitation of DNA content
Counterstain—ideal for use alongside other fluorophore reporters for fluorescence microscopy or high-content screening (HCS)

Applications:
• Flow cytometry
• Flow and laser scanning cytometry
• Live cell imaging
• Cell based assays

Properties of DRAQ5 Fluorescent Dye
• Structure: Proprietary
• Alternative names: Anthraquinone compound
• Chemical name(s): 1,5-bis{[2-(di-methylamino) ethyl]amino}-4, 8-dihydroxyanthracene-9,10-dione
• Molecular formula: Not available
• Molecular weight: 412.54
• Excitation wavelength: 647 nm line optimal (Exλmax = 646 nm)
• Emission wavelength: > 665 nm to infra-red > 800 nm (Emλmax = 681 nm/697 nm intercalated with dsDNA)
• Extinction coefficient: Not available
• CAS #: Not available
• Purity: > 99%
• Solubility: Soluble at working concentrations in water and typical buffers
• Reactive groups: None; binds DNA A-T minor groove, mimicking histone 2b distribution, resulting in 16 nm red-shift

Fluorescence cell-based assays, such as flow cytometry, in-cell ELISA, fluorescence microscopy and high-content imaging require a fluorescent label to identify individual cells. When using multiple fluorescent probes to detect different cellular targets or activities, each probe must have a fluorescent spectrum different than the other probes. The blue-fluorescent DNA-binding probes, Hoechst and DAPI are frequently used; however, these probes cannot be used when UV illumination is unavailable or other blue-emitting fluorescent probes are used. Therefore, nuclear probes that emit in a color other than blue are useful for cell identification and counting, and for determining nuclear morphology and DNA content.

DRAQ5 Fluorescent Probe emits in the far-red region, is lipophilic and crosses cell and nuclear membranes in live and fixed cells and tissues for rapid DNA staining. This stain is water-soluble, supplied ready to use and does not require RNase, cell lysis, or a washing step, making it compatible with automation. Because DNA staining is stoichiometric, the DRAQ5 Fluorescent Probe can be used for DNA content analysis in cell proliferation studies. DRAQ5 Fluorescent Probe is usually added as the last stain in a labeling procedure because no washing is required. Alternatively, add this probe in assay medium for a live cell assay.

Visualize staining your cell without wasting your reagents, antibodies, or time with our new Stain-iT Cell Staining Simulator.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Permeability
Cell-permeant
Color
Red
Description
Cellomics DRAQ5 Fluorescent Probe
Detection Method
Fluorescence
For Use With (Application)
Cell Imaging
For Use With (Equipment)
Fluorescence Microscope, High Content Analysis Instrument
Product Type
Fluorescent Probe Solution
Dye Type
Cell-Permeant
Emission
681 nm
Excitation Wavelength Range
647 nm
Form
Liquid
Product Line
Pierce™
Purity or Quality Grade
> 99%
Quantity
50 μL
Solubility
Soluble at working concentrations in water and typical buffers
Sub Cellular Localization
Nucleus, Cytosol, Cytoplasm

Contents & Storage

Store at 4°C.

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