DyLight™ 350 Antibody Labeling Kit

Catalog number: 62275

Thermo Scientific™  Related applications: Protein Labeling & Crosslinking

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The Thermo Scientific DyLight 350 Antibody Labeling Kit contains an NHS ester-activated derivative of high-performance DyLight 350 used to fluorescently label antibodies and other proteins that are then used as molecular probes for cellular imaging and other fluorescence detection methods. The standard size kit contains all necessary components to perform three separate labeling reactions using 1 mg of IgG or similar quantities of other proteins.

DyLight 350 is a derivative of aminomethylcoumarine acetate (AMCA) and has a higher fluorescence intensity than Alexa Fluor™ 350 and AMCA in many applications. The high water solubility of DyLight Fluors means that a high dye-to-protein ratio can be attained without causing precipitation of the conjugates. DyLight 350 Amine-Reactive Dye is also available as a stand-alone reagent.

Features of DyLight 350 NHS Ester:

High performance—DyLight 350 shows brighter fluorescence than Alexa Fluor 350 and AMCA
Specific—NHS ester-activated dye labels proteins and other molecules at primary amines (-NH2)
Convenient kit sizes—standard and microscale sizes are offered to match your experimental needs
Optimized procedure—following the standard protocol results in antibodies with excellent dye:protein ratios and recovery rates for optimum activity and fluorescence labeling

• Primary antibody labeling for immunofluorescence microscopy, immunohistochemistry (IHC), Western blotting or ELISA assay
• Target protein labeling for in vitro and in vivo fluorescent detection strategies

DyLight 350 Amine-Reactive Dye is activated with an N-hydroxysuccinimide (NHS) ester moiety to react with exposed N-terminal α-amino groups or the ε-amino groups of lysine residues to form stable amide bonds. Learn more about NHS ester chemistry.

Typical labeling reactions require DyLight 350 Amine-Reactive Dye to first be dissolved in anhydrous dimethyl formamide (DMF) or another suitable organic solvent before adding a specific molar amount of dye to an amine-free buffer containing the protein to be labeled. However, the high solubility of DyLight Fluors permits protein solutions to be added directly to specific amounts of the labeling reagent. This feature allows DyLight 350 Amine-Reactive Dye to be provided in multiple formats with flexible protocols to achieve efficient degrees of labeling.

Related Products
DyLight™ 350 NHS Ester
DyLight™ 350 Microscale Antibody Labeling Kit
For Research Use Only. Not for use in diagnostic procedures.


Label or Dye: DyLight™ 350
Product Size: 3 reactions
Labeling Scale: 0.5-1 mg
Labeling Target: Proteins (General), Antibodies (General)
Excitation⁄Emission (nm): 353⁄432

Contents & storage

• 3 vials DyLight NHS Ester
• 1 mL tube borate buffer
• 5 mL purification resin
• 6 spin columns
• 12 microcentrifuge collection tubes

Store the DyLight NHS Ester at -20°C. Store all other kit components at 4°C.


Manuals & protocols