Thermo Scientific™

Pierce™ G2 Fast Blotter

Catalog number: 62288
Thermo Scientific™

Pierce™ G2 Fast Blotter

Catalog number: 62288
Catalog Number
62288
Unit Size
1 unit
Price (USD)
Catalog NumberUnit SizePrice (USD)
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The Thermo Scientific Pierce G2 Fast Blotter is designed specifically for rapid semi-dry transfer of 10-300kDa proteins from polyacrylamide gels to nitrocellulose or PVDF membranes in 5 to 10 minutes.

Features of the G2 Fast Blotter:

Fast—transfer proteins in 5 to 10 minutes when used with 1-Step Transfer Buffer
Efficient—achieve high transfer efficiency with a broad range of protein sizes (10-300kDa) compared to conventional semi-dry or wet (tank) transfer methods
Integrated power supply—seamless operation between control unit and cassette provides consistent high efficiency protein transfer
Easy-touch programming—access pre-programmed transfer methods or create, save and run customized transfer methods
Convenient—simultaneously transfer 1 to 4 mini gels or 1 to 2 medium-sized gels
Versatile—use with Pierce 1-Step Transfer Buffer for rapid blotting programs or Towbin transfer buffer for conventional semi-dry transfer methods

The Pierce G2 Fast Blotter features an integrated power supply optimized to deliver consistent, high-efficiency protein transfer with commonly used pre-cast and homemade gels (SDS-PAGE) and nitrocellulose or PVDF membranes. The large cassette provides for simultaneous transfer of up to four mini-sized gels or two midi-sized gels. The blotter has an easy-to-use color LCD touchscreen interface and pre-programmed transfer methods for different numbers and sizes of gels and molecular weight ranges of proteins. The easy-touch programming feature allows custom transfer settings to be quickly created, saved and run.

The Pierce G2 Fast Blotter achieves highly efficient transfer in less than 10 minutes when used with Pierce 1-Step Transfer Buffer (Part No. 84731). The instrument is also effective for standard 30- to 60-minute semi-dry transfer protocols based on traditional buffers.

Includes:
• System consists of the Pierce G2 Fast Blotter Control Unit and Pierce G2 Fast Blotter Cassette; kit includes Western Blot Roller, Power Cord with C/13 Connector, and Quick Start Guide
• Unit ships with North American power cord; also available are country-specific power cords

Applications:
• Semi-dry Western blot transfer of 10-300kDa proteins from polyacrylamide gels to nitrocellulose or PVDF membranes

Traditional wet blotting techniques require transfer times of one hour to overnight to achieve good transfer efficiency. When used in conjunction with Pierce 1-Step Transfer Buffer, the Pierce G2 Fast Blotter is designed to provide transfer efficiency in 5-10 minutes that is equivalent, or better than, traditional transfer techniques for Western blotting. It also eliminates the need for gel pre-equilibration. This significant reduction in protein transfer time is accomplished by optimizing the ionic strength of the transfer buffer and increasing the current [amps (A)/cm2] flowing through the transfer stack. The Pierce G2 Fast Blotter can also be used for standard 30- to 60-minute semi-dry transfer protocols with Towbin buffer (Towbin et al., 1979).

 

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For Research Use Only. Not for use in diagnostic procedures.

Specifications

Contents & Storage

Important: locate the imager away from water, solvents, corrosive materials, strong magnetic fields and vibration sources.
Space Requirements:
The blotter requires a stable laboratory bench or table. Provide a minimum clearance of 3 inches (7.62cm) at the rear of the blotter to allow for adequate ventilation for cooling and access to the power cord and switch. In addition, allow sufficient space at the front of the blotter for docking and removing the cassette with/from the control unit.