DyLight™ 550-2xPEG Maleimide

Catalog number: 62292

Thermo Scientific™  Related applications: Protein Labeling & Crosslinking

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Description

Thermo Scientific DyLight 550-2xPEG Sulfhydryl-Reactive Dye is a maleimide-activated derivative of our high-performance DyLight 550 Dye used to fluorescently label cysteine-containing peptides, proteins or other biomolecular probes.

The DyLight 550-2xPEG Dye contains 2 polyethylene glycol (PEG) chains that are non-toxic, enhance fluorescence, and reduce nonspecific binding of conjugates made with them. Conjugates made with DyLight 550-2xPEG Dye can be used as molecular probes for cellular imaging, flow cytometry, and other fluorescence detection methods. The PEG chains also improve solubility of the dyes and labeled molecules in aqueous solution, aid in cell permeability, and improve tissue retention.

Features of DyLight 550-2xPEG Maleimide:

High fluorescence intensity—significantly brighter fluorescence than Alexa Fluor™ 555
PEGylated—improves solubility in aqueous solution and aids in cell permeability
Specific—maleimide-activated dye labels proteins and other molecules at reduced sulfhydryls (-SH)

Applications:
• Fluorescence microscopy
In vivo or ex vivo imaging
• Cell-based assays
• Flow cytometry/fluorescence-activated cell sorting (FACS)

DyLight 550-2xPEG Sulfhydryl-Reactive Dye is activated with a maleic acid imide (maleimide) moiety to form a reactive alkylation reagent. Labeling occurs through reaction of the maleimide-activated dye with reduced sulfhydryl groups (-SH) to form stable thioether bonds. Maleimides are specific for sulfhydryl groups between pH 6.5-7.5. Learn more about maleimide chemistry.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Solubility: DMF (Dimethylformamide)
Label or Dye: DyLight™ 550
Product Size: 1 mg
Chemical Reactivity: Thiol
Excitation⁄Emission (nm): 562⁄576

Contents & storage

Store desiccated at -20°C.

Documents

Manuals & protocols