Thermo Scientific™

DyLight™ 650-4xPEG Maleimide

Catalog number:  62294

 Related applications: Protein Labeling & Crosslinking

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Thermo Scientific DyLight 650-4xPEG Sulfhydryl-Reactive Dye is a maleimide-activated derivative of our high-performance DyLight 650 Dye used to fluorescently label cysteine-containing peptides, proteins, or other biomolecular probes.

The DyLight 650-4xPEG Dye contains 4 polyethylene glycol (PEG) chains that are non-toxic, enhance fluorescence, and reduce nonspecific binding of conjugates made with them. Conjugates made with DyLight 650-4xPEG Dye can be used as molecular probes for cellular and in vivo imaging, flow cytometry, IHC, and other fluorescence detection methods. The PEG chains also improve solubility of the dyes and labeled molecules in aqueous solution, aid in cell permeability, and improve tissue retention.

Features of DyLight 650-4xPEG Maleimide:

High fluorescence intensity—significantly brighter fluorescence than Alexa Fluor™ 647
PEGylated—improves solubility in aqueous solution and aids in cell permeability
Specific—maleimide-activated dye labels proteins and other molecules at reduced sulfhydryls (-SH)

• Fluorescence microscopy
In vivo or ex vivo imaging
• Cell-based assays
• Flow cytometry/fluorescence-activated cell sorting (FACS)

DyLight 650-4xPEG Sulfhydryl-Reactive Dye is activated with a maleic acid imide (maleimide) moiety to form a reactive alkylation reagent. Labeling occurs through reaction of the maleimide-activated dye with reduced sulfhydryl groups (-SH) to form stable thioether bonds. Maleimides are specific for sulfhydryl groups between pH 6.5–7.5. Learn more about maleimide chemistry.
For Research Use Only. Not for use in diagnostic procedures.


Chemical Reactivity: Thiol
Excitation⁄Emission (nm): 652⁄672
Label or Dye: DyLight™ 650
Product Line: DyLight™
Product Size: 1 mg
Solubility: DMF (Dimethylformamide)

Contents & storage

Store desiccated at -20°C.


Manuals & protocols

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