DyLight™ 650 Maleimide

Catalog number: 62295

Thermo Scientific™  Related applications: Protein Labeling & Crosslinking

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Description

Thermo Scientific DyLight 650 Sulfhydryl-Reactive Dye is a maleimide-activated derivative of high-performance DyLight 650 used to fluorescently label sulfhydryl-containing peptides, proteins, and other biomolecular probes.

DyLight 650 provides vibrant far-red fluorescence with comparable or improved performance over other dyes, including Alexa Fluor™ 647 and Cy5™ dye, over a broad pH range (pH 4-9). The high water solubility of DyLight Fluors means that a high dye-to-protein ratio can be attained without causing precipitation of the conjugates.

Features of DyLight 650 Maleimide:

High performance—DyLight 650 fluoresces brighter than Alexa Fluor 647 and Cy5
Specific—maleimide-activated dye labels proteins and other molecules at reduced sulfhydryls (-SH)
Efficient labeling methods—well-characterized chemistry and optimized protocols provide for reliable, high-quality labeling
Optimized antibody labeling procedure—complete protocol for IgG reduction and labeling and calculating the labeling efficiency

Applications:
• Antibody labeling for immunofluorescence applications, including immunocytochemistry (ICC), immunohistochemistry (IHC), Western blotting and ELISA assay
• Target macromolecule labeling for in vitro and in vivo fluorescent detection strategies

DyLight 650 Sulfhydryl-Reactive Dye is activated with a maleic acid imide (maleimide) moiety to form a reactive alkylation reagent. Labeling occurs through reaction of the maleimide-activated dye with reduced sulfhydryl groups (-SH) to form stable thioether bonds. Maleimides are specific for sulfhydryl groups between pH 6.5–7.5. Learn more about maleimide chemistry.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Chemical Reactivity: Thiol
Excitation⁄Emission (nm): 652⁄672
Label or Dye: DyLight™ 650
Product Line: DyLight™
Product Size: 1 mg
Solubility: DMF (Dimethylformamide)

Contents & storage

Store desiccated at -20°C. Shipped at ambient temperature.

Documents

Manuals & protocols