Invitrogen™

eBioscience™ JC-1 Mitochondrial Membrane Potential Dye

Catalog number: 65-0851-38
Invitrogen™

eBioscience™ JC-1 Mitochondrial Membrane Potential Dye

Catalog number: 65-0851-38
Catalog Number
65-0851-38
Unit Size
5 mg
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
65-0851-385 mg
-
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JC-1 is a membrane permeable dye widely used for determining mitochondrial membrane potential in flow cytometry and fluorescent microscopy. This dye can selectively enter the mitochondria where it reversibly changes color as membrane potentials increase (over values of about 80-100 mV). This property is due to the reversible formation of JC-1 aggregates upon membrane polarization that causes shifts in emitted light from 530 nm (i.e., emission of JC-1 monomeric form) to 590 nm (i.e., emission of J-aggregate) when excited at 488 nm. Both colors can be detected using filters for FITC and PE/phycoerythrin/rhodamine, respectively. JC-1 is both qualitative, in regards to the shift from green to orange fluorescence emission, and quantitative, as measured by fluorescence intensity, in both filter sets. JC-1 can be used to indicate the initiation of apoptosis.

Molecular Weight: 652.23
Peak Excitation: 515 nm
Peak Emission: 529 and 590nm

JC-1 should be reconstituted in high quality freshly opened DMSO at 1mg/mL. Once reconstituted, it should be stored at -20°C; avoid freeze-thawing.

Reported Application
Flow Cytometric Analysis, Microscopy

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Color
Orange
Detection Method
Fluorescent
For Use With (Equipment)
Fluorescence Microscope, Flow Cytometer
Product Type
Dye
Emission
515⁄529&590
Product Line
eBioscience™
Sub Cellular Localization
Mitochondria

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