eBioscience™ Calcein AM Viability Dye (UltraPure Grade)
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Invitrogen™

eBioscience™ Calcein AM Viability Dye (UltraPure Grade)

Calcein AM is a membrane-permeable live-cell labeling dye. Upon entering the cell, intracellular esterases cleave the acetoxymethyl (AM) ester group,Read more
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Catalog NumberQuantity
65-0853-7820 x 50 μg
65-0853-391 mg
Catalog number 65-0853-78
Price (USD)/ 20 x 50 µg
278.65
Online exclusive
300.00 
Save 21.35 (7%)
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Add to cart
Quantity:
20 x 50 μg
Price (USD)/ 20 x 50 µg
278.65
Online exclusive
300.00 
Save 21.35 (7%)
Add to cart
eBioscience™ Calcein AM Viability Dye (UltraPure Grade)
Catalog number65-0853-78
Price (USD)/ 20 x 50 µg
278.65
Online exclusive
300.00 
Save 21.35 (7%)
-
Add to cart
Calcein AM is a membrane-permeable live-cell labeling dye. Upon entering the cell, intracellular esterases cleave the acetoxymethyl (AM) ester group, yielding the membrane-impermeable Calcein fluorescent dye. Apoptotic and dead cells with compromised cell membranes do not retain Calcein. Calcein is optimally excited at 495 nm and has a peak emission of 515 nm. For fluorescent microscopy, Calcein can be detected using the appropriate filter sets. For flow cytometric analysis, it can be excited off the blue laser line (488 nm) and detected using filters for FITC/Alexa Fluor™ 488 (530/30). For multicolor flow cytometry, concentrations in the range of 5 – 20 nM are recommended to ensure that compensation can be performed successfully. Co-staining with Annexin V or 7-AAD is recommended to allow the greatest resolution between live and dead/apoptotic cells. If single-color analysis is desired, improved resolution between live and dead/apoptotic cells may be obtained by using either Calcein Blue AM (cat. 65-0855) or Calcein Violet 450 AM (cat. 65-0854).

Molecular weight: 994.86
Peak excitation: 495 nm
Peak emission: 515 nm

Calcein AM should be reconstituted in high-quality, freshly opened DMSO.
Once reconstituted, it should be stored at -20°C with dessicant and used within a short period of time. Avoid freeze-thawing.

Purity
>95% as determined by HPLC.

Reported Application
Flow Cytometric Analysis, Microscopy

For Research Use Only. Not for use in diagnostic procedures.
Specifications
ColorGreen
Detection MethodFluorescence
For Use With (Application)Viability Assay
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer
Product TypeDye
Emission495/515
Product LineeBioscience™
Quantity20 x 50 μg
Unit Size20 x 50 µg