Invitrogen™

eBioscience™ Fixable Viability Dye eFluor™ 450

Catalog number: 65-0863-14
Invitrogen™

eBioscience™ Fixable Viability Dye eFluor™ 450

Catalog number: 65-0863-14
Catalog Number
65-0863-14
Unit Size
100 tests
Price (USD)
105.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
500 tests
Price (USD)
296.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
65-0863-14100 tests
105.00
Your Price:
***
65-0863-18500 tests
296.00
Your Price:
***
Product Overview
Figures
Videos
Documents
FAQ
Citations & References
Additional Information
Recommendations
Fixable Viability Dye eFluor™ 450 is a viability dye that can be used to irreversibly label dead cells prior to cryopreservation, fixation and/or permeabilization procedures. Unlike 7-AAD and propidium iodide, cells labeled with Fixable Viability Dyes can be washed, fixed, permabilized, and stained for intracellular antigens without any loss of staining intensity of the dead cells. Thus, using Fixable Viability Dyes allows dead cells to be excluded from analysis when intracellular targets are being studied. Fixable Viability Dyes may be used to label cells from all species.

Fixable Viability Dye eFluor™ 450 can be excited by the violet (405 nm) laser line and has a peak excitation of 450 nm that can be detected using a 450/50 band pass filter (equivalent to eFluor™ 450 or Pacific Blue™). Please make sure that your instrument is capable of detecting this dye. For compensation, it is recommended to use a sample of the cells of interest stained with the Fixable Viability Dye. If the percentage of dead cells is expected to be less than 5%, then it is recommended to take a small aliquot of cells and heat them at 65°C for 1 minute then immediately place on ice for 1 minute. After this treatment, the heat-killed cells can be combined 1:1 with live cells and then stained with the Fixable Viability Dye.

Fixable Viability Dye eFluor™ 450 is supplied as a pre-diluted solution prepared in high-quality, anhydrous DMSO. It should be protected from light and moisture. Store at -70°C with dessicant. It may be freeze-thawed up to 20 times. Allow vial to equilibrate to room temperature before opening.

Reported Application
Flow Cytometric Analysis

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Color
Blue
Detection Method
Fluorescent
Emission
405⁄450
Product Line
eBioscience™
Quantity
100 tests
For Use With (Equipment)
Flow Cytometer

Figures

Frequently asked questions (FAQs)

Citations & References